Document Detail


Development and application of a loop-mediated isothermal amplification method on rapid detection Escherichia coli O157 strains from food samples.
MedLine Citation:
PMID:  19685165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed and evaluated the specificity and sensitivity of a loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Escherichia coli O157 strains. Six primers, including outer primers, inner primers and loop primers, were specially designed for recognizing eight distinct sequences on three targets, which were rfbE, stx1 and stx2. The detection limits were found to be 100, 100 and 10 fg DNA/tube for rfbE, stx1 and stx2, respectively. Application of LAMP assays were performed on 417 food-borne E. coli strains, the sensitivity of LAMP assays for the rfbE, stx1 and stx2 was 100, 95.3 and 96.3%, and the negative predictive value was 100, 96.7 and 97.1%, respectively; with a 100% specificity and positive predictive value for all three targets.
Authors:
Xihong Zhao; Yanmei Li; Li Wang; Lijun You; Zhenbo Xu; Lin Li; Xiaowei He; Yao Liu; Jihua Wang; Liansheng Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-15
Journal Detail:
Title:  Molecular biology reports     Volume:  37     ISSN:  1573-4978     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-09-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2183-8     Citation Subset:  IM    
Affiliation:
College of Light Industry and Food Sciences, South China University of Technology, 510640 Guangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Biological Assay
DNA Primers / metabolism
Escherichia coli O157 / genetics,  isolation & purification*
Food Analysis / methods*
Food Microbiology*
Polymerase Chain Reaction / methods*
Reference Standards
Sensitivity and Specificity
Temperature*
Chemical
Reg. No./Substance:
0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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