| Development and application of an enzymatic and cell flotation treatment for the recovery of viable microbial cells from environmental matrices such as anaerobic sludge. | |
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MedLine Citation:
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PMID: 22003005 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Efficient dissociation of microorganisms from their aggregate matrix is required to study the microorganisms without interaction with their native environment (e.g., biofilms, flocs, granules, etc.) and to assess their community composition through the application of molecular or microscopy techniques. To this end, we combined enzymatic treatments and a cell extraction by density gradient to efficiently recover anaerobic microorganisms from urban wastewater treatment plant sludge. The enzymes employed (amylase, cellulase, DNase, and pectinase) as a pretreatment softly disintegrated the extrapolymeric substances (EPS) interlocked with the microorganisms. The potential damaging effects of the applied procedure on bacterial and archaeal communities were assessed by studying the variations in density (using quantitative PCR), diversity (using capillary electrophoresis single-strand conformation polymorphism fingerprinting [CE-SSCP]), and activity (using a standard anaerobic activity test) of the extracted microorganisms. The protocol preserved the general capacity of the microbial community to produce methane under anaerobic conditions and its diversity; particularly the archaeal community was not affected in terms of either density or structure. This cell extraction procedure from the matrix materials offers interesting perspectives for metabolic, microscopic, and molecular assays of microbial communities present in complex matrices constituted by bioaggregates or biofilms. |
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Authors:
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Florence Braun; Jérôme Hamelin; Gaëlle Gévaudan; Dominique Patureau |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2011-10-14 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 77 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-02 Completed Date: 2012-03-21 Revised Date: 2012-06-19 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 8487-93 Citation Subset: IM |
Affiliation:
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INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, F-11100 Narbonne, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Archaea
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isolation & purification* Bacteria, Anaerobic / isolation & purification* Biodiversity Cell Survival Colony Count, Microbial DNA Fingerprinting Environmental Microbiology Enzymes / metabolism* Microbial Viability Microbiological Techniques / methods* Sewage / microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Enzymes; 0/Sewage |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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