| Development and application of different methods for the detection of Toxoplasma gondii in water. | |
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MedLine Citation:
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PMID: 12513983 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two methods, centrifugation and flocculation, were evaluated to determine their efficiencies of recovery of Toxoplasma gondii oocysts from contaminated water samples. Demineralized and tap water replicates were inoculated with high numbers of sporulated or unsporulated T. gondii oocysts (1 x 10(5) and 1 x 10(4) oocysts). The strain, age, and concentration of the seeded oocysts were recorded. Oocysts were recovered either by centrifugation of the contaminated samples at various g values or by flocculation with two coagulants, Fe(2)(SO(4))(3) and Al(2)(SO(4))(3). The recovery rates were determined with the final pellets by phase-contrast microscopy. Sporulated oocysts were recovered more effectively by flocculation with Al(2)(SO(4))(3) (96.5% +/- 21.7%) than by flocculation with Fe(2)(SO(4))(3) (93.1% +/- 8.1%) or by centrifugation at 2,073 x g (82.5% +/- 6.8%). For the unsporulated oocysts, flocculation with Fe(2)(SO(4))(3) was more successful (100.3% +/- 26.9%) than flocculation with Al(2)(SO(4))(3) (90.4% +/- 19.1%) or centrifugation at 2,565 x g (97.2% +/- 12.5%). The infectivity of the sporulated oocysts recovered by centrifugation was confirmed by seroconversion of all inoculated mice 77 days postinfection. These data suggest that sporulated Toxoplasma oocysts purified by methods commonly used for waterborne pathogens retain their infectivity after mechanical treatment and are able to induce infections in mammals. This is the first step in developing a systematic approach for the detection of Toxoplasma oocysts in water. |
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Authors:
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Christina Kourenti; Anja Heckeroth; Astrid Tenter; Panagiotis Karanis |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 69 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-01-06 Completed Date: 2003-03-21 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 102-6 Citation Subset: IM |
Affiliation:
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Institute for Medical Parasitology, Medical Faculty, University of Bonn, 53127 Bonn, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alum Compounds Animals Centrifugation Ferric Compounds Flocculation Tests Mice Oocysts / isolation & purification, pathogenicity Parasitology / methods Toxoplasma / growth & development, isolation & purification*, pathogenicity Toxoplasmosis, Animal / parasitology, physiopathology Water / parasitology* Water Pollution* Water Supply |
| Chemical | |
Reg. No./Substance:
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0/Alum Compounds; 0/Ferric Compounds; 10043-01-3/aluminum sulfate; 3HWS7HF5XD/ferric sulfate; 7732-18-5/Water |
| Comments/Corrections | |
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