Document Detail

Development and analytical application of an uric acid biosensor using an uricase-immobilized eggshell membrane.
MedLine Citation:
PMID:  17023154     Owner:  NLM     Status:  MEDLINE    
An uric acid biosensor fabricated from a uricase-immobilized eggshell membrane and an oxygen electrode was presented. The detection schemes involve the enzymatic reactions of the uricase leading to the depletion of dissolved oxygen level upon exposure to uric acid solution. The decrease in oxygen level was monitored and related to the uric acid concentration. The scanning electron micrographs show the microstructure of the eggshell membrane within which the uricase is successfully immobilized. The effects of enzyme loading, pH, temperature, and phosphate buffer concentration on the response of the biosensor were investigated in detail. The uric acid biosensor has a linear response range of 4.0-640 microM with a detection limit of 2.0 microM (S/N=3). The response time was less than 100 s. The biosensor exhibited good repeatable response to a 0.10mM uric acid solution with a relative standard deviation of 3.1% (n=7). The reproducibility of fabrication of the biosensors using four different membranes was good with a R.S.D. of 3.2%. The biosensor showed extremely good stability with a shelf-life of at least 3 months. Some common potential interferents in samples such as glucose, urea, ascorbic acid, lactic acid, glycine, DL-alpha-alanine, DL-cysteine, KCl, NaCl, CaCl2, MgSO4, and NH4Cl showed no interferences on the response of the uric acid biosensor. The biosensor was successfully applied to determine the uric acid level in some human serum and urine samples, and the results agreed well with those obtained by a commercial colorimetric assay kit.
Yan Zhang; Guangming Wen; Yehong Zhou; Shaomin Shuang; Chuan Dong; Martin M F Choi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-04
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  22     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-05     Completed Date:  2007-03-21     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1791-7     Citation Subset:  IM    
Institute of Advanced Chemistry, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
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MeSH Terms
Biosensing Techniques / methods*
Egg Shell
Enzymes, Immobilized / metabolism*
Hydrogen-Ion Concentration
Urate Oxidase / metabolism*
Uric Acid / analysis*
Reg. No./Substance:
0/Buffers; 0/Enzymes, Immobilized; 69-93-2/Uric Acid; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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