Document Detail


Development and validation of a real-time TaqMan assay for the detection and enumeration of Pseudomonas fluorescens ATCC 13525 used as a challenge organism in testing of food equipments.
MedLine Citation:
PMID:  22250861     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PRACTICAL APPLICATION: Pseudomonas fluorescens ATCC 13525 is used as a challenge organism in the efficacy testing of clean-in-place process of food equipments. Currently, culturing technique is used for its identification and estimation, which is not only time-consuming but also prone to error. Real-time TaqMan assay is more specific, sensitive, and accurate along with a shorter turnaround time compared to culturing techniques, thereby increasing the overall quality of the testing methodology to evaluate the clean-in-place process critical for the food industry to protect public health and safety.
Authors:
Ratul Saha; Lorelle L Bestervelt; Robert S Donofrio
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2012-01-17
Journal Detail:
Title:  Journal of food science     Volume:  77     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-20     Completed Date:  2012-06-18     Revised Date:  2013-07-15    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M150-5     Citation Subset:  IM    
Copyright Information:
© 2012 Institute of Food Technologists®
Affiliation:
Dept of Microbiology and Molecular Biology, NSF Intl, 789 N Dixboro Rd, Ann Arbor, MI 48105, USA. rsaha@nsf.org
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial
DNA Primers / genetics
DNA, Bacterial / isolation & purification*
Food Contamination / analysis*
Food Handling / methods
Food Microbiology / methods*
Pseudomonas fluorescens / genetics,  growth & development,  isolation & purification*
Real-Time Polymerase Chain Reaction / methods*
Sequence Analysis, DNA
Software
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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