Document Detail

Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species.
MedLine Citation:
PMID:  22891917     Owner:  NLM     Status:  Publisher    
Abstract Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.
Silvina P Brengi; Stephen B O'Brien; Mariana Pichel; Carol Iversen; Matthew Arduino; Norma Binsztein; Bette Jensen; Franco Pagotto; Efrain M Ribot; Roger Stephan; Nicole Cernela; Kara Cooper; Séamus Fanning
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-14
Journal Detail:
Title:  Foodborne pathogens and disease     Volume:  -     ISSN:  1556-7125     ISO Abbreviation:  Foodborne Pathog. Dis.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120121     Medline TA:  Foodborne Pathog Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1 Servicio Enterobacterias, Departamento Bacteriología, INEI-ANLIS "Dr. Carlos G. Malbrán," Buenos Aires, Argentina .
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