Document Detail


Development of a UHPLC-ESI-MS/MS Method for the Simultaneous Determination of Salicylic Acid, Jasmonic Acid and Abscisic Acid in Rose Leaves.
MedLine Citation:
PMID:  24932512     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This paper describes a method to detect and quantitate the endogenous plant hormones (±)-2-cis-4-trans-abscisic acid, (-)-jasmonic acid and salicylic acid by means of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in hybrid rose leaf matrices. Deuterium-labeled [(2)H6] (+)-2-cis-4-trans-abscisic acid, [(2)H6] (±)-jasmonic acid, and [(2)H4]-salicylic acid were used as internal standards. Rose samples (10 mg) were extracted with methanol/water/acetic acid (10:89:1) and subsequently purified on an Oasis MCX 1cc Vac SPE Cartridge. Performance characteristics were validated according to Commission Decision 2002/657/EC.(28) Recovery, repeatability and within-laboratory reproducibility were acceptable for all phytohormones tested at three different concentrations. The decision limit and detection capability for (±)-2-cis-4-trans-abscisic acid, (-)-jasmonic acid and salicylic acid were 0.0075 µg/g and 0.015 µg/g, 0.00015 µg/g and 0.00030 µg/g and 0.0089 µg/g and 0.018 µg/g, respectively. Matrix effects (signal suppression or enhancement) appeared to be high for all substances considered implying the need for quantitation based on matrix-matched calibration curves.
Authors:
Renato Bosco; Els Daeseleire; Els Van Pamel; Valentina Scariot; Leen Leus
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-16
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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