| Development of tissue-engineered human periodontal ligament constructs with intrinsic angiogenic potential. | |
| | |
MedLine Citation:
|
PMID: 19365111 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
One approach to treat periodontal diseases is grafting of tissue-engineered periodontal ligaments. Therefore, periodontal ligaments were constructed by layering cell sheets. A cell sheet was prepared by enzymatic digestion of salmon collagen gel on which human periodontal ligament fibroblasts (HPLFs) were co-cultured with or without human umbilical vein endothelial cells (HUVECs). Three cell sheets were layered and then cultured in angiogenic media, in which the HUVECs were found to form capillary-like structures when co-cultured on the HPLFs. The layered HPLFs sheets with HUVEC co-culture (PL-EC construct) demonstrated longer survival, higher alkaline phosphatase activities and lower osteocalcin production than layered HPLFs sheets without HUVEC co-culture (PL construct). Hematoxylin-eosin and Masson's trichrome staining of histological sections showed that cell density, mass and extracellular matrix deposition of the PL-EC construct were higher than those of the PL construct. Furthermore, CD31 immunostaining revealed the formation of capillary-like structures throughout the PL-EC construct. In conclusion, we successfully developed tissue-engineered periodontal ligament constructs with intrinsic angiogenic potential using cell sheet engineering and HUVEC co-culture. |
| | |
Authors:
|
Nobuhiro Nagai; Ayumi Hirakawa; Nao Otani; Masanobu Munekata |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-10 |
Journal Detail:
|
Title: Cells, tissues, organs Volume: 190 ISSN: 1422-6421 ISO Abbreviation: Cells Tissues Organs (Print) Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-11-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883360 Medline TA: Cells Tissues Organs Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 303-12 Citation Subset: IM |
Copyright Information:
|
2009 S. Karger AG, Basel. |
Affiliation:
|
Creative Research Initiative 'Sousei', Hokkaido University, Sapporo, Japan. nagai491@yahoo.co.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regeneration of a Spinal Ligament after Total Lumbar Disk Arthroplasty in Primates.
Next Document: The effects of intra-aortic balloon pump support on macrocirculation and tissue microcirculation in ...