Document Detail

Development and Survival of Orius insidiosus (Say) Nymphs on Encapsulated Bee Pollen-Based Diet in a Tier-I Toxicity Assay.
MedLine Citation:
PMID:  22217780     Owner:  NLM     Status:  In-Data-Review    
The insidious flower bug, Orius insidiosus (Say) (Heteroptera: Anthocoridae) is an important surrogate species for assessing potential effects of plant-incorporated protectants (PIPs) on nontarget heterotrophic predators. In this study, a continuous dietary exposure system was optimized by assessing the effect of diet composition and age on the survival and development of nymphs of O. insidiosus. Greater than 85% control survival and an acceptable rate of development from nymph hatching to adult was achieved using 5-d-old nymphs at test initiation and a bee pollen-based diet supplemented with 25% Ephestia eggs. There was an unacceptable level of mortality (>40%) and/or a significantly prolonged development time when nymphs were <5 d old at test initiation. When 5-d-old nymphs were fed a bee pollen diet containing 25% Ephestia eggs and 100 μg/g potassium arsenate, time-dependent mortality was observed with a median lethal time (LT(50)) of 4.4 d and 100% mortality was observed after 10 d of feeding, indicating the effectiveness of the test system to detect adverse effects by dietary exposure. It is recommended that well-defined 5-d-old nymphs and an encapsulated bee pollen-based diet containing 25% ground Ephestia eggs be used in a Tier-I dietary feeding exposure assay for detecting potential effects of PIPs on O. insidiosus nymphs.
Jianguo Tan; Mark S Paradise; Steven L Levine; Pamela M Bachman; Joshua P Uffman; Changjian Jiang; David B Carson
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Environmental entomology     Volume:  40     ISSN:  1938-2936     ISO Abbreviation:  Environ. Entomol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-01-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502320     Medline TA:  Environ Entomol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1613-21     Citation Subset:  IM    
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