Document Detail

Development of a stable isotope dilution analysis for the quantification of the Bacillus cereus toxin cereulide in foods.
MedLine Citation:
PMID:  19994891     Owner:  NLM     Status:  MEDLINE    
An increasing number of severe food borne intoxications are caused by a highly stable depsipeptide, named cereulide, which is produced by emetic Bacillus cereus strains. As cereulide poses a health risk to humans, the development of an appropriate method for the analysis of this toxin is mandatory. Therefore, the reference material of cereulide as well as its (13)C(6)-isotopologue was prepared by means of a biosynthetic approach using a B. cereus culture, followed by a rapid but efficient downstream purification. After structure confirmation by means of liquid chromatography (LC)-time-of-flight mass spectrometry, LC-tandem mass spectrometry, and one-/two-dimensional NMR spectroscopy, a stable isotope dilution analysis (SIDA) was developed for the quantification of cereulide in foods using the (13)C(6)-cereulide as the internal standard. Validation experiments were performed, and the data were compared to the quantitative analysis using the structurally related valinomycin instead of the (13)C(6)-cereulide as an internal standard. Trueness, repeatability, and reproducibility expressed as relative standard deviation showed values <10 or <8% for valinomycin or <8% for (13)C(6)-cereulide, respectively. Furthermore, the MS response of the valinomycin was found to be significantly influenced by the food matrix, thus leading to rather low recovery rates of 91% from boiled rice and 80% from boiled rice supplemented with 10% sunflower oil. In comparison, the use of (13)C(6)-cereulide as an internal standard gave good recovery rates of 104 and 111% from both matrices, thus demonstrating the robustness and accuracy of the developed SIDA.
Tobias Bauer; Timo Stark; Thomas Hofmann; Monika Ehling-Schulz
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1420-8     Citation Subset:  IM    
Microbiology Unit, ZIEL Nutrition and Food Research Center, WZW, Technische Universitat Munchen, 85354 Freising, Germany.
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MeSH Terms
Bacillus cereus / chemistry*,  metabolism
Bacterial Toxins / analysis*,  metabolism
Carbon Isotopes / analysis*,  metabolism
Depsipeptides / analysis*,  metabolism
Food Contamination / analysis*
Isotope Labeling / methods*
Reg. No./Substance:
0/Bacterial Toxins; 0/Carbon Isotopes; 0/Depsipeptides; 0/cereulide

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