Document Detail

Development of Sertoli cell binding competency in the peripubertal rat.
MedLine Citation:
PMID:  9796617     Owner:  NLM     Status:  MEDLINE    
The peripheral distribution of Sertoli cell F-actin, a cytoskeletal protein found in Sertoli cell ectoplasmic specializations, is associated with enhanced spermatid binding to the Sertoli cell and, as such, serves as a functional marker for its acquisition of binding competency. Previous studies suggest that the peripheral distribution of actin is dependent on follicle-stimulating hormone (FSH). To investigate the developmental pattern of Sertoli cell actin distribution in relation to peripubertal FSH and testosterone levels, we examined epithelial sheets from 2-8 week-old-rats. Tissues were processed for light microscopy and for the visualization of rhodamine-labeled F-actin. At 2 weeks, actin staining was diffuse throughout most of the Sertoli cells and was similar to that observed in binding-incompetent Sertoli cells. By 4 weeks, actin distribution was peripheral, acquiring the same staining pattern as observed in binding-competent Sertoli cells. Serum levels of FSH peaked at 4 weeks and declined to adult levels thereafter. Testosterone levels did not increase significantly until 6 weeks. Results show that Sertoli cell actin undergoes peripheral reorganization concurrent with the peripubertal peak of FSH but prior to the peripubertal rise of testosterone. The study demonstrates a temporal correlation between the peripubertal FSH rise and the actin redistribution in Sertoli cells that is consistent with an induction of this redistribution by FSH. These results suggest that FSH induces binding competency in Sertoli cells.
D F Cameron; K E Muffly; S J Nazian
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of andrology     Volume:  19     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    1998 Sep-Oct
Date Detail:
Created Date:  1998-12-14     Completed Date:  1998-12-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  573-9     Citation Subset:  IM    
Department of Anatomy, University of South Florida College of Medicine, Tampa 33612, USA.
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MeSH Terms
Actins / metabolism
Cell Adhesion*
Follicle Stimulating Hormone / blood
Sertoli Cells / cytology*
Sexual Maturation*
Testis / cytology,  growth & development,  metabolism
Testosterone / blood
Reg. No./Substance:
0/Actins; 58-22-0/Testosterone; 9002-68-0/Follicle Stimulating Hormone

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