Document Detail


Development of Sertoli cell binding competency in the peripubertal rat.
MedLine Citation:
PMID:  9796617     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The peripheral distribution of Sertoli cell F-actin, a cytoskeletal protein found in Sertoli cell ectoplasmic specializations, is associated with enhanced spermatid binding to the Sertoli cell and, as such, serves as a functional marker for its acquisition of binding competency. Previous studies suggest that the peripheral distribution of actin is dependent on follicle-stimulating hormone (FSH). To investigate the developmental pattern of Sertoli cell actin distribution in relation to peripubertal FSH and testosterone levels, we examined epithelial sheets from 2-8 week-old-rats. Tissues were processed for light microscopy and for the visualization of rhodamine-labeled F-actin. At 2 weeks, actin staining was diffuse throughout most of the Sertoli cells and was similar to that observed in binding-incompetent Sertoli cells. By 4 weeks, actin distribution was peripheral, acquiring the same staining pattern as observed in binding-competent Sertoli cells. Serum levels of FSH peaked at 4 weeks and declined to adult levels thereafter. Testosterone levels did not increase significantly until 6 weeks. Results show that Sertoli cell actin undergoes peripheral reorganization concurrent with the peripubertal peak of FSH but prior to the peripubertal rise of testosterone. The study demonstrates a temporal correlation between the peripubertal FSH rise and the actin redistribution in Sertoli cells that is consistent with an induction of this redistribution by FSH. These results suggest that FSH induces binding competency in Sertoli cells.
Authors:
D F Cameron; K E Muffly; S J Nazian
Related Documents :
3005317 - Rat sertoli cells secrete a growth factor that blocks epidermal growth factor (egf) bin...
6086557 - Role of the pineal in the alteration of hamster sertoli cell responsiveness to fsh duri...
10952937 - Effects of thyroid and luteinizing hormones on the onset of precursor cell differentiat...
14600347 - Spermatogenesis, serum testosterone levels and immunolocalization of steroidogenic enzy...
17127417 - The role of b cells in animal models of rheumatoid arthritis.
11251067 - Phosphatidylcholine synthesis influences the diacylglycerol homeostasis required for se...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of andrology     Volume:  19     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    1998 Sep-Oct
Date Detail:
Created Date:  1998-12-14     Completed Date:  1998-12-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  573-9     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of South Florida College of Medicine, Tampa 33612, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Cell Adhesion*
Follicle Stimulating Hormone / blood
Male
Rats
Sertoli Cells / cytology*
Sexual Maturation*
Spermatogenesis
Testis / cytology,  growth & development,  metabolism
Testosterone / blood
Chemical
Reg. No./Substance:
0/Actins; 58-22-0/Testosterone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Müllerian inhibiting substance improves longevity of motility and viability of fresh and cryopreserv...
Next Document:  Unilateral injection of neuropeptide Y decreases blood flow in the injected testis but may also incr...