Document Detail


Development of a PDMS-based microchip electrophoresis device for continuous online in vivo monitoring of microdialysis samples.
MedLine Citation:
PMID:  20358542     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A PDMS-based microfluidic system for online coupling of microdialysis sampling to microchip electrophoresis with fluorescence detection for in vivo analysis of amino acid neurotransmitters using naphthalene-2,3-dicarboxaldehyde and sodium cyanide as the derivatization reagents is described. Fabricating chips from PDMS rather than glass was found to be simpler and more reproducible, especially for chips with complex designs. The microchip incorporated a 20-cm serpentine channel in which sample plugs were introduced using a "simple" injection scheme; this made fluid handling and injection on-chip easier for the online system compared with gated or valve-based injection. The microchip was evaluated offline for the analysis of amino acid standards and rat brain microdialysis samples. Next, precolumn derivatization was incorporated into the chip and in vivo online microdialysis-microchip electrophoresis studies were performed. The system was employed for the continuous monitoring of amino acid neurotransmitters in the extracellular fluid of the brain of an anesthetized rat. Fluorescein was dosed intravenously and monitored simultaneously online as a marker of in vivo blood-brain barrier permeability. The microdialysis-microchip electrophoresis system described here will be employed in the future for simultaneous monitoring of changes in blood-brain barrier permeability and levels of amino acid neurotransmitters in the rat stroke model.
Authors:
Pradyot Nandi; Dhara P Desai; Susan M Lunte
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  31     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-27     Completed Date:  2010-07-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1414-22     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Animals
Brain Chemistry
Dimethylpolysiloxanes / chemistry*
Electrophoresis, Microchip / instrumentation*,  methods*
Fluorescein / chemistry
Hydrogen-Ion Concentration
Microdialysis / methods*
Naphthalenes / chemistry
Neurotransmitter Agents / analysis*
Nylons / chemistry*
Rats
Rats, Sprague-Dawley
Sodium Cyanide / chemistry
Spectrometry, Fluorescence / methods
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Dimethylpolysiloxanes; 0/Naphthalenes; 0/Neurotransmitter Agents; 0/Nylons; 0/poly(dimethylsiloxane)-polyamide copolymer; 143-33-9/Sodium Cyanide; 2321-07-5/Fluorescein; 7149-49-7/2,3-naphthalenedicarboxaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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