Document Detail

Development of a PCR protocol to detect aflatoxigenic molds in food products.
MedLine Citation:
PMID:  22221359     Owner:  NLM     Status:  In-Data-Review    
Aflatoxins are secondary metabolites produced mainly by Aspergillus species growing in foodstuffs. Because aflatoxins have important health effects, the detection of early contamination of foods by aflatoxigenic molds should be useful. In the present work, a reliable conventional PCR method for detecting aflatoxigenic molds of various species was developed. Fifty-six aflatoxigenic and nonaflatoxigenic strains commonly reported in foodstuffs were tested. Aflatoxin production was first confirmed by micellar electrokinetic capillary electrophoresis or/and high-pressure liquid chromatography-mass spectrometry. Based on the conserved regions of the O-methyltransferase gene (omt-1) involved in the aflatoxin biosynthetic pathway, six primer pairs were designed. With only the designed primer pair AFF1-AFR3, the expected PCR product (381 bp) was obtained in all of the tested aflatoxigenic strains of various species and genera. Amplification products were not obtained with this primer pair for any of the nonaflatoxigenic reference molds. However, an amplicon of 453 bp was obtained for all aflatoxigenic and nonaflatoxigenic mold reference strains with a PCR protocol based on the constitutive fungal β-tubulin gene, which was used as a positive fungal control. The PCR protocol based on omt-1 detected as little as 15 pg of DNA from aflatoxigenic molds and 10(2) to 10(3) CFU/g in contaminated food samples. This PCR protocol should be used as a routine technique to detect aflatoxigenic molds in foods.
M Isabel Luque; Alicia Rodríguez; María J Andrade; Alberto Martín; Juan J Córdoba
Related Documents :
8240869 - Double-barrelled tubular duplication of the ileum.
2751329 - Management of oesophageal stenosis in epidermolysis bullosa dystrophica.
21871969 - Glucosensing and glucose homeostasis: from fish to mammals.
21664709 - Emerging roles of engineered nanomaterials in the food industry.
21761979 - Regulating genetically modified food.
3921329 - Enzymatic digestion of esophageal meat impaction. a study of adolph's meat tenderizer.
6145139 - Dynorphin: important mediator for electroacupuncture analgesia in the spinal cord of th...
9816489 - Utilization of nitrogen and macro-minerals in response to nutritional status in clinica...
23768359 - Isolation and characterization of a cyanidin-catechin pigment from adzuki bean (vigna a...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  75     ISSN:  1944-9097     ISO Abbreviation:  J. Food Prot.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  85-94     Citation Subset:  IM    
Higiene y Seguridad Alimentaria, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad s/n, 10003-Cáceres, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Incidence of Salmonella, Listeria monocytogenes, Escherichia coli O157:H7, and Staphylococcal Entero...
Next Document:  Microbial performance of food safety management systems implemented in the lamb production chain.