Document Detail


Development of an optimized ELISA and a sample preparation method for the detection of beta-lactoglobulin traces in baby foods.
MedLine Citation:
PMID:  20073478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dairy products can induce allergic reactions even when present at very low levels, such as levels found in involuntary contamination during food manufacturing. beta-Lactoglobulin (betaLG) is the main allergen in cow's milk. The objective of this work was to develop a sensitive method for betaLG detection in baby foods through the optimization of an innovative sample preparation method. Three types of baby foods deliberately contaminated with dairy products or dairy desserts were sterilized to simulate the potential contamination occurring during manufacturing and then used as samples. Different sample preparation methods were compared. The best results were provided by an extraction solution containing beta-mercaptoethanol, guanidine hydrochloride, and a saline solution. An ELISA method was optimized for the detection of betaLG (LOD = 9.7 x 10(-13) M). The developed method allowed detection of even 1 part of dairy product in 100,000 parts of baby food for some of the analyzed foods.
Authors:
Cristina Pelaez-Lorenzo; Jose Carlos Diez-Masa; Isabel Vasallo; Mercedes de Frutos
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1664-71     Citation Subset:  IM    
Affiliation:
Institute of Organic Chemistry, Juan de la Cierva 3, 28006 Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Enzyme-Linked Immunosorbent Assay / methods*
Humans
Infant
Infant Food / analysis*
Lactoglobulins / analysis*
Milk / chemistry
Chemical
Reg. No./Substance:
0/Lactoglobulins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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