Document Detail

Development of Oligoclonal Nanobodies for Targeting the Tumor-Associated Glycoprotein 72 Antigen.
MedLine Citation:
PMID:  23015323     Owner:  NLM     Status:  Publisher    
The tumor-associated glycoprotein 72 (TAG-72) is a membrane mucin whose over-expression is correlated with advanced tumor stage and increased invasion and metastasis. In this study, we identified a panel of four nanobodies, single variable domains of dromedary heavy-chain antibodies that specifically recognize the TAG-72 antigen. All selected nanobodies were shown to selectively bind to this cancer-related molecule with low-nanomolar affinities and do not cross-react with other antigens, such as MUC1 or HER2. Furthermore, they can detect TAG-72 in concentrations as low as 5 U/ml which is valuable in sensitive detection of this molecule in cancerous patients. Cell ELISA experiments proved their ability for binding to the native target antigen on TAG-72 expressing cells while not showing any reactivity to HT-29 cells, a TAG-72-negative cell line. Using competition studies, we found that each nanobody recognizes a distinct epitope on the TAG-72 antigen that is different from the one recognized by the mouse anti-TAG-72 antibody, CC49. Considering their high specificity, reduced immunogenicity and multi-targeting behavior, these oligoclonal nanobodies represent a promising tool to target TAG-72 over-expressing tumor cells.
Zahra Sharifzadeh; Fatemeh Rahbarizadeh; Mohammad Ali Shokrgozar; Davoud Ahmadvand; Fereidoun Mahboudi; Fatemeh Rahimi Jamnani; Seyed Hamid Aghaee Bakhtiari
Related Documents :
9202313 - Demyelinating antibodies to myelin oligodendrocyte glycoprotein and galactocerebroside ...
8842023 - Morphological changes at paranodes in igm paraproteinaemic neuropathy.
23529913 - The effects of interleukin-6 signal blockade on immune system, reproductive and skeleta...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Molecular biotechnology     Volume:  -     ISSN:  1559-0305     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Vitamin D and the racial difference in the genotype 1 chronic hepatitis C treatment response.
Next Document:  Tired of Diabetes Genetics? Circadian Rhythms and Diabetes: The MTNR1B Story?