Document Detail


Development of a Novel PCR-ELISA Assay for the Diagnosis of Trichophyton rubrum Onychomycosis.
MedLine Citation:
PMID:  23301809     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: The prevalence of onychomycosis has increased steadily in the last decade. An accurate diagnosis at the outset is important for successful and cost-effective treatment of patients. However, current diagnostic tests for onychomycosis are neither rapid nor sensitive nor specific. OBJECTIVES: Therefore, we developed a microsatellite-based PCR-ELISA (MS-ELISA) assay for the detection of Trichophyton rubrum, which is the most common etiologic agent of onychomycosis. METHODS: An archival set of 434 nail and skin specimens from 217 patients was included as the test sample in this study. We also compared MS-ELISA with an earlier published topoisomerase PCR-ELISA (TI-ELISA) assay using template DNA extracted by another method. RESULT: MS-ELISA detected highest number of positive samples (69%) followed by direct microscopy (56%), TI-ELISA (44%) and fungal culture (30%). When an identical DNA extraction method was applied to 120 specimens, MS-ELISA proved to be twice as sensitive as TI-ELISA. In conclusion, we have optimized a target gene and DNA extraction method for rapid detection of T. rubrum onychomycosis.
Authors:
F Pankewitz; P Nenoff; S Uhrlaß; G Bezold; I Winter; Y Gräser
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  The British journal of dermatology     Volume:  -     ISSN:  1365-2133     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
©The Authors bjd © 2013 British Association of Dermatologists.
Affiliation:
Konsiliarlabor für Dermatophyten, Institut für Mikrobiologie und Hygiene, Universitätsmedizin - Berlin Charité, Berlin, Germany.
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