Document Detail

Development of a modified Caco-2 cell model system for studying iron availability in eggs.
MedLine Citation:
PMID:  20170171     Owner:  NLM     Status:  MEDLINE    
A modified Caco-2 cell model system was developed for studying iron availability in mixtures of fresh and/or cooked foods subjected to a simulated gastrointestinal digestion. The effect of combining foods containing high levels of ascorbic acid with cooked eggs on ferritin expression in the cells was measured. There was no detectable increase in ferritin with eggs alone, indicating that none of the iron was available for uptake into the cells, but when mixed with orange juice or salad (lettuce, tomatoes, and red pepper) in ratios similar to those found in meals, there was a significant increase in ferritin concentration (p = 0.0012 and p = 9.2 x 10(-10), respectively); the enhancing effect of salad was greater than orange juice (p = 0.028). These results suggest that the iron in eggs will be more readily absorbed when consumed with foods high in ascorbic acid.
Ben Thompson; Paul Sharp; Ruan Elliott; Sharifah Al-Mutairi; Susan J Fairweather-Tait
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3833-9     Citation Subset:  IM    
School of Medicine, University of East Anglia, Norwich, United Kingdom.
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MeSH Terms
Biological Availability
Caco-2 Cells
Eggs / analysis*
Iron, Dietary / pharmacokinetics*
Models, Biological
Grant Support
//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Iron, Dietary

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