| Development of a Listeria monocytogenes EGDe partial proteome reference map and comparison with the protein profiles of food isolates. | |
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MedLine Citation:
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PMID: 12788738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A partially annotated proteome reference map of the food pathogen Listeria monocytogenes was developed for exponentially growing cells under standardized, optimal conditions by using the sequenced strain EGDe (serotype 1/2a) as a model organism. The map was developed by using a reproducible total protein extraction and two-dimensional (2-D) polyacrylamide gel electrophoresis analysis procedure, and it contained 33 identified proteins representing the four main protein functional classes. In order to facilitate analysis of membrane proteins, a protein compartmentalization procedure was assessed. The method used provided partial fractionation of membrane and cytosolic proteins. The total protein 2-D profiles of three serotype 1/2a strains and one serotype 1/2b strain isolated from food were compared to the L. monocytogenes EGDe proteome. An average of 13% of the major protein spots in the food strain proteomes were not matched in the strain EGDe proteome. The variation was greater for the less intense spots, and on average 28% of these spots were not matched. Two of the proteins identified in L. monocytogenes EGDe were missing in one or more of the food isolates. These two proteins were proteins involved in the main glycolytic pathway and in metabolism of coenzymes and prosthetic groups. The two corresponding genes were found by PCR amplification to be present in the four food isolates. Our results show that the L. monocytogenes EGDe reference map is a valuable starting point for analyses of strains having various origins and could be useful for analyzing the proteomes of different isolates of this pathogen. |
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Authors:
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Manilduth Ramnath; K Björn Rechinger; Lothar Jänsch; John W Hastings; Susanne Knøchel; Anne Gravesen |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 69 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-06-05 Completed Date: 2003-10-01 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3368-76 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Stellenbosch, 7602 Matieland, South Africa. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Proteins / genetics, isolation & purification, metabolism* Computational Biology Electrophoresis, Gel, Two-Dimensional / methods Humans Listeria monocytogenes / classification*, genetics, growth & development, metabolism* Meat / microbiology Peptide Mapping Proteome* Reference Standards Serotyping |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Proteome |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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