| Development of a label free IGE sensitive aptasensor based on electrochemical impedance spectrometry. | |
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MedLine Citation:
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PMID: 20426746 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5' Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analysis are based on electron transfer resistance (Rct) in the presence of 5mM [Fe(CN)6]3-/4-. |
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Authors:
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Pinar Kara; Buket Meric; Mehmet Ozsoz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Combinatorial chemistry & high throughput screening Volume: 13 ISSN: 1875-5402 ISO Abbreviation: Comb. Chem. High Throughput Screen. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2011-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810948 Medline TA: Comb Chem High Throughput Screen Country: Netherlands |
Other Details:
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Languages: eng Pagination: 578-81 Citation Subset: IM |
Affiliation:
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Department of Analytical Chemistry, Ege University, Bornova, Izmir, Turkey. pinar.kara@ege.edu.tr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aptamers, Nucleotide
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chemistry* Biosensing Techniques / instrumentation, methods* Coated Materials, Biocompatible / chemistry Electric Impedance Electrochemistry Graphite / chemistry High-Throughput Screening Assays / instrumentation, methods Humans Immunoglobulin E / analysis* Sensitivity and Specificity Streptavidin / chemistry Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 0/Coated Materials, Biocompatible; 37341-29-0/Immunoglobulin E; 7782-42-5/Graphite; 9013-20-1/Streptavidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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