Document Detail

Development of a LC-MS/MS method for the determination of antrodin B and antrodin C from Antrodia camphorata extract in rat plasma for pharmacokinetic study.
MedLine Citation:
PMID:  20542396     Owner:  NLM     Status:  MEDLINE    
A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of antrodin B and antrodin C in rat plasma. Both target compounds, together with the internal standard (diazepam), were extracted from rat plasma samples by liquid-liquid extraction with ethyl acetate. Chromatographic separation was carried out on an Agilent XDB-C(8) column with an isocratic mobile phase consisting of acetonitrile and water (70:30, V/V) at a flow rate of 0.5 mL/min. The mass spectrometric detection was performed by selected reaction monitoring (SRM) mode via atmospheric pressure chemical ionization (APCI) source operating in positive ionization mode. The assay exhibited a linear dynamic range of 47.6-4760 ng/mL for antrodin B and 56.6-5660 ng/mL for antrodin C. The intra- and inter-day precision was less than 5.3% and the accuracy was less than 2.7% for both analytes. The validated method has been applied to the pharmacokinetic study of antrodin B and antrodin C in rats following oral administration of Antrodia camphorata extract.
Yongli Liu; Xin Di; Xingchao Liu; Wenjin Shen; Kelvin Sze-Yin Leung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-12
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  53     ISSN:  1873-264X     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-09-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  781-4     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier B.V. All rights reserved.
School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.
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MeSH Terms
Antrodia / chemistry*
Chromatography, Liquid / methods*
Maleimides / blood*,  pharmacokinetics
Plant Extracts / analysis*
Rats, Sprague-Dawley
Tandem Mass Spectrometry / methods*
Reg. No./Substance:
0/Maleimides; 0/Plant Extracts; 0/antrodin B; 0/antrodin C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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