| Development of an improved protocol for the isolation and detection of Enterobacter sakazakii (Cronobacter) from powdered infant formula. | |
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MedLine Citation:
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PMID: 20537255 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F chromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii. |
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Authors:
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Yi Chen; Kwang-Yong Song; Eric W Brown; Keith A Lampel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of food protection Volume: 73 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-11 Completed Date: 2010-07-08 Revised Date: 2011-08-02 |
Medline Journal Info:
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Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
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Languages: eng Pagination: 1016-22 Citation Subset: IM |
Affiliation:
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U.S. Food and Drug Administration, 5100 Paint Branch Parkway HFS-711, College Park, Maryland 20740, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Consumer Product Safety DNA, Bacterial / analysis* Enterobacter sakazakii / isolation & purification* Food Contamination / analysis* Humans Infant Infant Food / microbiology* Infant Formula Infant, Newborn Polymerase Chain Reaction / methods* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
| Comments/Corrections | |
Comment In:
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J Food Prot. 2010 Nov;73(11):1964-6; author reply 1965-6
[PMID:
21219706
]
J Food Prot. 2011 Jun;74(6):872-3 [PMID: 21669061 ] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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