Document Detail

Development of an improved protocol for the isolation and detection of Enterobacter sakazakii (Cronobacter) from powdered infant formula.
MedLine Citation:
PMID:  20537255     Owner:  NLM     Status:  MEDLINE    
Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F chromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.
Yi Chen; Kwang-Yong Song; Eric W Brown; Keith A Lampel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  73     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-11     Completed Date:  2010-07-08     Revised Date:  2011-08-02    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1016-22     Citation Subset:  IM    
U.S. Food and Drug Administration, 5100 Paint Branch Parkway HFS-711, College Park, Maryland 20740, USA.
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MeSH Terms
Consumer Product Safety
DNA, Bacterial / analysis*
Enterobacter sakazakii / isolation & purification*
Food Contamination / analysis*
Infant Food / microbiology*
Infant Formula
Infant, Newborn
Polymerase Chain Reaction / methods*
Reg. No./Substance:
0/DNA, Bacterial
Comment In:
J Food Prot. 2010 Nov;73(11):1964-6; author reply 1965-6   [PMID:  21219706 ]
J Food Prot. 2011 Jun;74(6):872-3   [PMID:  21669061 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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