| Development of an H(2)(15)O steady-state method combining a bolus and slow increasing injection with a multiprogramming syringe pump. | |
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MedLine Citation:
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PMID: 20664614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An (15)O-labeled water (H(2)(15)O) steady-state method for quantitative measurement of cerebral blood flow (CBF), which is less stressful to small animals with a few point blood sampling, was developed. After a simulation using a dose meter to achieve stable H(2)(15)O radioactivity in the blood with a multiprogramming syringe pump programmed for slowly increasing injection volume, 10 rats were studied with the injection method. Arterial blood was sampled every minute during 6-minute positron emission tomography (PET) scans. After the PET scan, N-isopropyl-p-[(125)I]-iodoamphetamine ((125)I-IMP) was injected into the same rat to measure CBF using the autoradiography method based on a microsphere model. Regions of interest were placed on the whole brain in H(2)(15)O-PET and (125)I-IMP-autoradiography images, and CBF values calculated from both methods were compared. Radioactivity in the dose meter achieved equilibrium ∼1 minute after starting the H(2)(15)O injection. In rat studies, radioactivity in the blood and brain rapidly achieved equilibrium at 2 minutes after administration. The correlation of CBF values of H(2)(15)O PET (49.2±5.4 mL per 100 g per minute) and those of (125)I-IMP autoradiography (49.1±5.2 mL per 100 g per minute) was excellent (y=1.01x-0.37, r(2)=0.97). The H(2)(15)O steady-state method with a continuously increasing injection is useful for CBF measurement in small animal studies, especially when multiple scans are required in the same animal. |
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Authors:
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Masato Kobayashi; Yasushi Kiyono; Rikiya Maruyama; Tetsuya Mori; Keiichi Kawai; Hidehiko Okazawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-28 |
Journal Detail:
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Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 31 ISSN: 1559-7016 ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-04 Completed Date: 2011-03-17 Revised Date: 2012-02-01 |
Medline Journal Info:
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Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: United States |
Other Details:
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Languages: eng Pagination: 527-34 Citation Subset: IM |
Affiliation:
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Biomedical Imaging Research Center, University of Fukui, Fukui, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Autoradiography Blood Gas Analysis Brain / radionuclide imaging* Cerebrovascular Circulation / physiology* Data Interpretation, Statistical Iofetamine / diagnostic use Isotope Labeling Male Microspheres Oxygen Radioisotopes / diagnostic use Positron-Emission Tomography Radiopharmaceuticals / diagnostic use Rats Rats, Sprague-Dawley Syringes Water / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Oxygen Radioisotopes; 0/Radiopharmaceuticals; 7732-18-5/Water; 95896-48-3/Iofetamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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