| Development of an engraftable skin equivalent based on matriderm with human keratinocytes and fibroblasts | |
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MedLine Citation:
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PMID: 19711256 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A cell-based wound coverage with keratinocytes and fibroblasts on the basis of a commercially available dermal substitute (Matriderm ((R)), Kollagen/Elastin matrix) was generated, in order to treat wide burn wounds. First the expansion of keratinocytes was optimised and the culturing time was minimised. Raw material was 1-2 cm (2) split skin. Dermis and epidermis were separated by enzymatic treatment with thermolysin. After treatment of both compartments with trypsin and collagenase I, keratinocytes and fibroblasts were isolated and expanded in collagen I coated dishes. After 10 days fibroblasts were seeded on Matriderm ((R)). After cultivation of the fibroblasts-containing matrix for one week keratinocytes were seeded on top. After an additional week of submersed cultivation the matrix was lifted up to the air-liquid interface to initiate epidermal cell differentiation. After 16 days in the air-liquid interphase the matrix was fixed and underwent immunohistochemical and electron microscopic analysis. Histological analysis showed a regularly stratification of the epidermal part. We observed collagen IV, a marker for the basement membrane, between epidermis and dermis. Desmoglein and the differentiation markers involucrine and cytokeratin 10 were found in the suprabasal layers of the epidermis. Electron microscopic analysis showed the basement membrane in the epidermal junction zone as well as cell-cell connections in the form of desmosomes. Late differentiation characteristics, like granular structures and the cornified layer, were found in the stratum granulosum and stratum corneum. Our results demonstrate that a skin equivalent can be generated by using a collagen/elastin matrix, with an expansion rate of 50-100-fold. This skin equivalent may be useful for covering deep wounds. |
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Authors:
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P A Golinski; N Z?ller; S Kippenberger; H Menke; J Bereiter-Hahn; A Bernd |
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Publication Detail:
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Type: English Abstract; Journal Article Date: 2009-08-26 |
Journal Detail:
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Title: Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft f?r Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft f?r Mikrochirurgie der Peripheren Nerven und Gef?sse : Organ der Vereinigung der Deutschen Plastischen Chirurgen Volume: 41 ISSN: 1439-3980 ISO Abbreviation: Handchir Mikrochir Plast Chir Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-21 Completed Date: 2010-03-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8302815 Medline TA: Handchir Mikrochir Plast Chir Country: Germany |
Other Details:
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Languages: ger Pagination: 327-32 Citation Subset: IM |
Affiliation:
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Zentrum der Dermatologie und Venerologie, Goethe-Universit?t, Frankfurt/M. |
Vernacular Title:
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Entwicklung eines transplantierbaren Haut?quivalentes auf Basis von Matriderm mit menschlichen Keratinozyten und Fibroblasten. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Basement Membrane
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pathology Burns / pathology, surgery* Collagen* / ultrastructure Collagen Type IV / analysis Desmogleins / analysis Elastin* / ultrastructure Epidermis / pathology Fibroblasts / pathology, transplantation* Humans Keratinocytes / pathology, transplantation* Microscopy, Electron Microscopy, Fluorescence Protein Precursors / analysis Skin / pathology Skin, Artificial* Tissue Engineering* |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type IV; 0/Desmogleins; 0/Protein Precursors; 0/matriderm; 60108-77-2/involucrin; 9007-34-5/Collagen; 9007-58-3/Elastin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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