| Development of a D-alanine sensor for the monitoring of a fermentation using the improved selectivity by the combination of D-amino acid oxidase and pyruvate oxidase. | |
| | |
MedLine Citation:
|
PMID: 14623466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A D-alanine (D-Ala) sensor for the monitoring of a fermentation process was developed using flow injection analysis (FIA). The FIA system consisted of a D-amino acid oxidase (D-AAOx) reactor, a Pyruvate oxidase (PyOx) electrode and a contrast electrode in the flow cell, and through the oxidation of D-amino acids in the D-AAOx reactor, pyruvic acid was formed only from D-Ala. The pyruvic acid was further oxidized with PyOx via the D-AAOx reaction. The amount of oxygen consumed in the PyOx reaction was proportional to the amount of D-Ala. It was possible to continuously repeat the assay up to 60 times at pH 6.8 and a flow rate of 0.18-ml min(-1). A linear relationship was obtained in the range of 0.1-1 mM D-Ala with a correlation coefficient of 0.987 and the detection limit was 0.05 mM. The relative standard deviation (R.S.D.) was 4.9% (n=5) for 0.5 mM D-Ala. The D-Ala content in some fish sauces was also determined using the proposed sensor system. The results obtained indicated a linear relationship between the amounts of D-Ala determined by the proposed sensor system and the conventional method. From the results, even if the substrate specificity of the enzyme (D-AAOx) was low, it was evident that the concentration of the original material (D-Ala) could be determined specifically when the first reaction product was changed by the second reaction (PyOx). |
| | |
Authors:
|
Yohei Inaba; Kentaro Mizukami; Naoko Hamada-Sato; Takeshi Kobayashi; Chiaki Imada; Etsuo Watanabe |
Related Documents
:
|
18760386 - Snake venomics of the brazilian pitvipers bothrops cotiara and bothrops fonsecai. ident... 21191576 - Clinical significance of uric acid dihydrate in urinary stones. 12162546 - Cloning and expression of fructosyl-amino acid oxidase gene from corynebacterium sp. 2-... 3146976 - Spectroscopic studies of the reaction between bovine serum amine oxidase (copper-contai... 15596476 - Metabolic profiling of medicago truncatula cell cultures reveals the effects of biotic ... 864236 - Peroxisomes in pulmonate gastropods. |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article; Validation Studies |
Journal Detail:
|
Title: Biosensors & bioelectronics Volume: 19 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2003 Dec |
Date Detail:
|
Created Date: 2003-11-18 Completed Date: 2004-08-09 Revised Date: 2009-07-14 |
Medline Journal Info:
|
Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
|
Languages: eng Pagination: 423-31 Citation Subset: IM |
Affiliation:
|
Department of Food Science and Technology, Tokyo University of Fisheries, 5-7 Kounan 4, Minato-ku, Tokyo 108-8477, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alanine
/
analysis*,
chemistry* Biosensing Techniques / instrumentation*, methods Cell Culture Techniques / instrumentation*, methods D-Amino-Acid Oxidase / chemistry* Electrochemistry / instrumentation*, methods Equipment Design Equipment Failure Analysis Flow Injection Analysis / instrumentation*, methods Online Systems Pyruvate Oxidase / chemistry* Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
|
56-41-7/Alanine; EC 1.2.3.3/Pyruvate Oxidase; EC 1.4.3.3/D-Amino-Acid Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Directed immobilization of nucleic acids at ultramicroelectrodes using a novel electro-deposited pol...
Next Document: Electrochemiluminescent biosensors array for the concomitant detection of choline, glucose, glutamat...