Document Detail

Development of a closed chest model of chronic myocardial infarction in Swine: magnetic resonance imaging and pathological evaluation.
MedLine Citation:
PMID:  24282645     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Our aim was to develop an easy-to-induce, reproducible, and low mortality clinically relevant closed-chest model of chronic myocardial infarction in swine using intracoronary ethanol and characterize its evolution using MRI and pathology. We injected 3-4 mL of 100% ethanol into the mid-LAD of anesthetized swine. Heart function and infarct size were assessed serially using MRI. Pigs were euthanized on days 7, 30, and 90 (n = 5 at each timepoint). Postoperative MRI revealed compromised contractility and decreased ejection fraction, from 53.8% ± 6.32% to 43.79% ± 7.72% (P = 0.001). These values remained lower than baseline thorough the followup (46.54% ± 11.12%, 44.48% ± 7.77%, and 40.48% ± 6.40%, resp., P < 0.05). Progressive remodeling was seen in all animals. Infarcted myocardium decreased on the first 30 days (from 18.09% ± 7.26% to 9.9% ± 5.68%) and then stabilized (10.2% ± 4.21%). Pathology revealed increasing collagen content and fibrous organization over time, with a rim of preserved endocardial cells. In conclusion, intracoronary ethanol administration in swine consistently results in infarction. The sustained compromise in heart function and myocardial thinning over time indicate that the model may be useful for the preclinical evaluation of and training in therapeutic approaches to heart failure.
Verónica Crisóstomo; Juan Maestre; Manuel Maynar; Fei Sun; Claudia Báez-Díaz; Jesús Usón; Francisco M Sánchez-Margallo
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Publication Detail:
Type:  Journal Article     Date:  2013-10-27
Journal Detail:
Title:  ISRN cardiology     Volume:  2013     ISSN:  2090-5580     ISO Abbreviation:  ISRN Cardiol     Publication Date:  2013  
Date Detail:
Created Date:  2013-11-27     Completed Date:  2013-11-27     Revised Date:  2014-01-24    
Medline Journal Info:
Nlm Unique ID:  101566293     Medline TA:  ISRN Cardiol     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  781762     Citation Subset:  -    
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