Document Detail

Development of Chitosan Oligosaccharide-Modified Gold Nanorods for In vivo Targeted Delivery and Non-Invasive Imaging by NIR Irradiation.
MedLine Citation:
PMID:  23030814     Owner:  NLM     Status:  Publisher    
In the present study, we demonstrate the synthesis and applications of multifunctional gold nanorod-based probes for specific targeting and non-invasive imaging based on localized heating generated by gold nanorods after NIR irradiation. The structural design of the probe consists of MUA (11-mercaptoundecanoic acid)-capped gold nanorods covalently linked with low molecular weight chitosan oligosaccharide (Mw~5000) via carbo-diimide (EDC) coupling agent. This surface modification is performed for complete replacement of toxic CTAB (hexadecyltrimethyl-ammonium chloride) and acid-responsive delivery of gold nanorods in acidic environment as known to be present at tumor surrounding areas. The resulting chitosan oligosaccharide-modified gold nanorods (CO-GNRs) were further conjugated with tumor targeting monoclonal antibody against EGFR (epidermal growth factor receptor) to provide localized targeting functionality owing to the over expression of EGFR in human oral adenosquamous carcinoma cell line CAL 27. Initial in vitro and in vivo toxicity assessments indicated that CO-GNRs did not induce any significant toxicity and are thus suitable for biological applications. Furthermore, selective targeting and accumulation of CO-GNRs were observed in vitro via two-photon luminescence imaging studies in CAL 27, which was also observed through in vivo targeting studies performed via NIR (near infrared) laser irradiation in CAL 27 xenografts of BALB/c nude mice. Hence, the CO-GNRs that we have developed are biocompatible, non-toxic and can be a potential candidate for in vivo targeted delivery, non-invasive imaging based on localized hyperthermia and photothermal-related therapies.
Charan Shobhit; Kumar Sanjiv; Narendra Singh; Fan-Ching Chien; Yi-Fan Chen; Navchtsetseg Nergui; Shih-Hsin Huang; Chiung Wen Kuo; Te-Chang Lee; Peilin Chen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-3
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  -     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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