Document Detail


Development and certification of a standard reference material for vitamin D metabolites in human serum.
MedLine Citation:
PMID:  22141317     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health's Office of Dietary Supplements (NIH-ODS), has developed a Standard Reference Material (SRM) for the determination of 25-hydroxyvitamin D [25(OH)D] in serum. SRM 972 Vitamin D in Human Serum consists of four serum pools with different levels of vitamin D metabolites and has certified and reference values for 25(OH)D(2), 25(OH)D(3), and 3-epi-25(OH)D(3). Value assignment of this SRM was accomplished using a combination of three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). Chromatographic resolution of the 3-epimer of 25(OH)D(3) proved to be essential for accurate determination of the metabolites.
Authors:
Karen W Phinney; Mary Bedner; Susan S-C Tai; Veronica V Vamathevan; Lane C Sander; Katherine E Sharpless; Stephen A Wise; James H Yen; Rosemary L Schleicher; Madhulika Chaudhary-Webb; Christine M Pfeiffer; Joseph M Betz; Paul M Coates; Mary Frances Picciano
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-12-28
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-23     Completed Date:  2012-05-15     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  956-62     Citation Subset:  IM    
Affiliation:
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States. karen.phinney@nist.gov
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid
Humans
Mass Spectrometry
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Vitamin D / analogs & derivatives*,  analysis,  blood,  standards
Grant Support
ID/Acronym/Agency:
Y01 OD000183/OD/NIH HHS; Y01 OD009087/OD/NIH HHS
Chemical
Reg. No./Substance:
1406-16-2/Vitamin D; 64719-49-9/25-hydroxyvitamin D
Comments/Corrections

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