Document Detail

Development of Bacteriostatic DNA Aptamers for Salmonella.
MedLine Citation:
PMID:  23387511     Owner:  NLM     Status:  Publisher    
Salmonella is one of the most dangerous and common foodborne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Aptamers have grown in popularity since their discovery and their properties make them attractive candidates for therapeutic use. In this work, we describe the selection of highly specific DNA aptamers to S. enteritidis and S. typhimurium. To evolve species-specific aptamers, twelve rounds of selection to live S. enteritidis and S. typhimurium were performed, alternating with a negative selection against a mixture of related pathogens. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential.
Olga S Kolovskaya; Anna G Savitskaya; Tatiana N Zamay; Irina T Reshetneva; Galina S Zamay; Evgeny N Erkaev; Xiaoyan Wang; Mohamed Wehbe; Alla B Salmina; Olga V Perianova; Olga A Zubkova; Ekaterina A Spivak; Vasily S Mezko; Yury E Glazyrin; Nadezhda M Titova; Maxim V Berezovski; Anna S Zamay
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-7
Journal Detail:
Title:  Journal of medicinal chemistry     Volume:  -     ISSN:  1520-4804     ISO Abbreviation:  J. Med. Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9716531     Medline TA:  J Med Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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