Document Detail

Development and application of reverse transcription loop-mediated isothermal amplification for detecting live Shewanella putrefaciens in preserved fish sample.
MedLine Citation:
PMID:  22515249     Owner:  NLM     Status:  MEDLINE    
PRACTICAL APPLICATION: The study provided a rapid and accurate detection method for live bacteria in aquatic food and established a new procaryotic mRNA isolation strategy at the same time, which will be useful for food preservation.
Chenghua Li; Qi Ying; Xiurong Su; Taiwu Li
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food science     Volume:  77     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-20     Completed Date:  2012-08-14     Revised Date:  2014-11-13    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M226-30     Citation Subset:  IM    
Copyright Information:
© 2012 Institute of Food Technologists®
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MeSH Terms
Base Sequence
DNA, Bacterial / chemistry,  metabolism
DNA, Complementary / metabolism
DNA, Intergenic / chemistry,  metabolism
Electrophoresis, Agar Gel
Food Inspection / methods*
Food, Preserved / microbiology*
Hot Temperature
Molecular Sequence Data
Nucleic Acid Amplification Techniques*
Perciformes / microbiology*
Poly A / metabolism
RNA, Bacterial / isolation & purification,  metabolism
RNA, Messenger / isolation & purification,  metabolism
RNA-Directed DNA Polymerase / metabolism
Seafood / microbiology*
Shewanella putrefaciens / genetics,  isolation & purification*,  metabolism
Time Factors
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Complementary; 0/DNA, Intergenic; 0/RNA, Bacterial; 0/RNA, Messenger; 24937-83-5/Poly A; EC DNA Polymerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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