Document Detail


Development and application of an HILIC-MS/MS method for the quantitation of nucleotides in infant formula.
MedLine Citation:
PMID:  20726584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the quantitation of nucleotides (adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP), uridine 5'-monophosphate (UMP), cytidine 5'-monophosphate (CMP), and inosine 5'-monophosphate (IMP)) in infant formula was developed by hydrophilic interaction chromatography with tandem mass spectrometry (HILIC-MS/MS). The internal standards used (AMP-13C10, 15N5; GMP-13C10, 15N5; UMP-13C9, 15N2; CMP-13C9, 15N3) were prepared with centrifugal ultrafiltration (CUF). Data acquisition was achieved by using multiple reaction monitoring (MRM) of product ions of protonated molecules of the five nucleotides generated by the positive-ion ESI. HILIC conditions were performed with 30 mmol/L ammonium formate in water (pH 2.5, adjusted with formic acid) and methanol. The LOD and LOQ were 5-10 μg/mL and 10-30 μg/mL for standard solution, respectively. Recovery for intra- and interday assays ranged from 98.1 to 108.9% (RSD: 0.7-5.4%) spiked with three concentration levels (5, 25, and 250 μg/g powder infant formula). This method could be applied for the determination of nucleotides in infant formula samples. The detected concentrations of five nucleotides ranged from not detected (n.d.) to 278 μg/g powder infant formula. The total nucleotide level ranged from n.d. to 600.2 μg/g powder infant formula.
Authors:
Koichi Inoue; Rutsuko Obara; Tomoaki Hino; Hisao Oka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-15     Completed Date:  2011-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9918-24     Citation Subset:  IM    
Affiliation:
Department of Physical and Analytical Chemistry, School of Pharmacy, Kinjo Gakuin University, Japan. kinoue@kinjo-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Food Analysis / methods*
Humans
Infant
Infant Formula / chemistry*
Nucleotides / analysis*,  chemistry
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Chemical
Reg. No./Substance:
0/Nucleotides

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