Document Detail


Developing a novel fiber optic fluorescence device for multiplexed high-throughput cytotoxic screening.
MedLine Citation:
PMID:  20734922     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The need for new pharmacological agents is unending. Yet the drug discovery process has changed substantially over the past decade and continues to evolve in response to new technologies. There is presently a high demand to reduce discovery time by improving specific lab disciplines and developing new technology platforms in the area of cell-based assay screening. Here we present the developmental concept and early stage testing of the Ab-Sniffer, a novel fiber optic fluorescence device for high-throughput cytotoxicity screening using an immobilized whole cell approach. The fused silica fibers are chemically functionalized with biotin to provide interaction with fluorescently labeled, streptavidin functionalized alginate-chitosan microspheres. The microspheres are also functionalized with Concanavalin A to facilitate binding to living cells. By using lymphoma cells and rituximab in an adaptation of a well-known cytotoxicity protocol we demonstrate the utility of the Ab-Sniffer for functional screening of potential drug compounds rather than indirect, non-functional screening via binding assay. The platform can be extended to any assay capable of being tied to a fluorescence response including multiple target cells in each well of a multi-well plate for high-throughput screening.
Authors:
Dennis Lee; Stephen Barnes
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of experimental therapeutics & oncology     Volume:  8     ISSN:  1359-4117     ISO Abbreviation:  J. Exp. Ther. Oncol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-25     Completed Date:  2010-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9604933     Medline TA:  J Exp Ther Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  235-45     Citation Subset:  IM    
Affiliation:
mAbGen LLC., R&D Department, 1250 Wood Branch Park Dr, Suite 625, Houston, TX 77079, USA. dlee@mabgenllc.com
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MeSH Terms
Descriptor/Qualifier:
Drug Discovery
Drug Screening Assays, Antitumor / methods*
Fiber Optic Technology / instrumentation*
Fluorescence
High-Throughput Screening Assays / methods*
Humans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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