| Developing a new clinical tool for diagnosing chronic Q fever: the Coxiella ELISPOT. | |
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MedLine Citation:
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PMID: 22067057 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Definitively establishing a clinical diagnosis of chronic Q fever remains challenging as the diagnostic performance of both conventional serological tests and PCR is limited. Given the importance of an early diagnosis of chronic Q fever there is a need for a reliable diagnostic test. We developed an enzyme-linked immunospot assay to measure Coxiella burnetii (C. burnetii) specific T-cell responses (Coxiella ELISPOT) to both phase I and phase II antigens and tested convalescent Q fever patients (without chronic disease, n=9) and patients with an established diagnosis of chronic Q fever (n=3). The Coxiella ELISPOT adequately identified convalescent Q fever patients from healthy controls by demonstrating C. burnetii specific T-cell interferon-γ production to both phase I and phase II antigens. Compared to convalescent Q fever patients, chronic Q fever patients showed a distinct Coxiella ELISPOT profile characterized by a much higher spot count for both phase I and phase II (18-fold for phase II, 8-fold higher for phase I) and a consistent shift towards more phase I reactivity. The diagnostic potential of the Coxiella ELISPOT is promising and warrants further investigation. |
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Authors:
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Gjm Limonard; Sf Thijsen; Aw Bossink; A Asscheman; Jjm Bouwman |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-3 |
Journal Detail:
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Title: FEMS immunology and medical microbiology Volume: - ISSN: 1574-695X ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9315554 Medline TA: FEMS Immunol Med Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Department of Pulmonary Diseases, Diakonessenhuis Utrecht, The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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