Document Detail


Deuterium isotope effect in gamma-aminobutyric acid transamination: determination of rate-limiting step.
MedLine Citation:
PMID:  3794716     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rate of transamination of gamma-aminobutryic acid (GABA) catalyzed by hog brain gamma-aminobutyrate aminotransferase was substantially reduced when the hydrogen at the gamma-carbon position was replaced by deuterium. The deuterium isotope effect of this reaction has been substantiated by fluorometric, radiometric, and mass spectrometric procedures and assessed kinetically. The ratios of Vmax of the nonlabeled substrate/Vmax of the deuterated substrate obtained under different conditions ranged from 6 to 7. This indicates that the cleavage of the hydrogen from the gamma-carbon is the rate-determining step in GABA transamination. Similar isotope effects have also been shown to occur in the peripheral system in vivo.
Authors:
P H Yu; D A Durden; B A Davis; A A Boulton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  48     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-02-19     Completed Date:  1987-02-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  440-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
4-Aminobutyrate Transaminase / metabolism
Animals
Brain / enzymology
Chemical Phenomena
Chemistry
Deuterium / metabolism*
Fluorometry
Kinetics
Male
Mass Spectrometry
Rats
Rats, Inbred Strains
Swine
gamma-Aminobutyric Acid / metabolism*
Chemical
Reg. No./Substance:
56-12-2/gamma-Aminobutyric Acid; 7782-39-0/Deuterium; EC 2.6.1.19/4-Aminobutyrate Transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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