| Detoxified extract of Rhus verniciflua stokes inhibits rotenone-induced apoptosis in human dopaminergic cells, SH-SY5Y. | |
| | |
MedLine Citation:
|
PMID: 21061154 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Rhus verniciflua Stokes (RVS), traditionally used as a food supplement and in traditional herbal medicine for centuries in Korea, is known to possess various pharmacological properties. Environmental neurotoxins such as rotenone, a specific inhibitor of complex I provide models of Parkinson's disease (PD) both in vivo and in vitro. In this study, we investigated the neuroprotective effect of RVS against rotenone-induced toxicity in human dopaminergic cells, SH-SY5Y. Cells exposed to rotenone for 24 h-induced cellular injury and apoptotic cell death. Pretreatment of cells with RVS provided significant protection to SH-SY5Y cells. Further, RVS offered remarkable protection against rotenone-induced oxidative stress and markedly inhibited mitochondrial membrane potential (MMP) disruption. RVS also attenuated the up-regulation of Bax, Caspase-9 and Caspase-3 and down-regulation of Bcl-2. Moreover, pretreatment with RVS prevented the decrease in tyrosine hydroxylase (TH) levels in SH-SY5Y cells. Interestingly, RVS conferred profound protection to human dopaminergic cells by preventing the downregulation of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). These results suggest that RVS may protect dopaminergic neurons against rotenone-induced apoptosis by multiple functions and contribute to neuroprotection in neurodegenerative diseases, such as PD. |
| | |
Authors:
|
Kumar Sapkota; Seung Kim; Se-Eun Park; Sung-Jun Kim |
Related Documents
:
|
12875694 - The role of nf-kappab in hepatocellular carcinoma cell. 10093644 - Distribution of apoptosis-related proteins in the quail ovary during folliculogenesis: ... 18534994 - The developing human ovary: immunohistochemical analysis of germ-cell-specific vasa pro... 11739184 - Arsenic trioxide induces apoptosis in human t-cell leukemia virus type 1- and type 2-in... 17965154 - Characterization of nucleotide pools as a function of physiological state in escherichi... 12847144 - Changes in human bladder epithelial cell gene expression associated with interstitial c... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cellular and molecular neurobiology Volume: 31 ISSN: 1573-6830 ISO Abbreviation: Cell. Mol. Neurobiol. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-05-02 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8200709 Medline TA: Cell Mol Neurobiol Country: United States |
Other Details:
|
Languages: eng Pagination: 213-23 Citation Subset: IM |
Affiliation:
|
Department of Biotechnology, Chosun University, Gwang-ju, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expressions of per1 clock gene and genes of signaling peptides vasopressin, vasoactive intestinal pe...
Next Document: Localization of prohibitin in the nuclear matrix and alteration of its expression during differentia...