Document Detail


Detoxification of endotoxin by endodontic irrigants and calcium hydroxide.
MedLine Citation:
PMID:  11485249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of endodontic irrigants and calcium hydroxide on lipopolysaccharide (LPS; endotoxin) were analyzed using the highly selective technique of mass spectrometry/gas chromatography with selected ion monitoring. An aqueous solution of LPS was mixed with one of a variety of endodontic irrigants for 30 min. Because it is a commonly used interappointment dressing, calcium hydroxide was also applied to LPS for 1, 2, or 5 days. LPS inactivation was measured by quantitation of free fatty acid release. Water, EDTA, ethanol, 0.12% chlorhexidine, chlorhexidine + sodium hypochlorite, and sodium hypochlorite alone showed little breakdown of LPS. Long-term calcium hydroxide--as well as 30-min exposure to an alkaline mixture of chlorhexidine, ethanol, and sodium hypochlorite--did detoxify LPS molecules by hydrolysis of ester bonds in the fatty acid chains of the lipid A moiety.
Authors:
R A Buck; J Cai; P D Eleazer; R H Staat; H E Hurst
Related Documents :
18539809 - Variations in the degree of d-alanylation of teichoic acids in lactococcus lactis alter...
3125849 - Isolation of type 5 capsular polysaccharide from staphylococcus aureus.
19438019 - Influence of nitric acid treatment of carbon nanotubes on their physico-chemical proper...
8119289 - Characterization of the lipopolysaccharide of moraxella catarrhalis. structural analysi...
17236019 - Analysis of hydrosoluble polysaccharides from ramonda myconi leaves.
7729869 - Detection of muramic acid in a carbohydrate fraction of human spleen.
2442419 - The effect of some urinary stone inhibitors on membrane interaction potentials of stone...
20006519 - A boronic acid chalcone analog of combretastatin a-4 as a potent anti-proliferation agent.
9034219 - In vitro human skin barrier modulation by fatty acids: skin permeation and thermal anal...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endodontics     Volume:  27     ISSN:  0099-2399     ISO Abbreviation:  J Endod     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-08-03     Completed Date:  2001-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  325-7     Citation Subset:  D    
Affiliation:
University of Louisville, KY, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques
Calcium Hydroxide / pharmacology*
Chromatography, Gas
Endotoxins / antagonists & inhibitors*,  chemistry
Ion-Selective Electrodes
Lipid A / analysis,  chemistry
Lipolysis
Lipopolysaccharides / chemistry*
Mass Spectrometry
Myristic Acids / analysis,  chemistry
Polysaccharides, Bacterial / drug effects*
Root Canal Irrigants / pharmacology*
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Lipid A; 0/Lipopolysaccharides; 0/Myristic Acids; 0/Polysaccharides, Bacterial; 0/Root Canal Irrigants; 1305-62-0/Calcium Hydroxide; 1961-72-4/beta-hydroxymyristic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evaluation of regional bond strength of resin cement to endodontic surfaces.
Next Document:  Antimicrobial activity of Ca(OH)2 containing pastes with Enterococcus faecalis in vitro.