| Detoxification of endotoxin by endodontic irrigants and calcium hydroxide. | |
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MedLine Citation:
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PMID: 11485249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of endodontic irrigants and calcium hydroxide on lipopolysaccharide (LPS; endotoxin) were analyzed using the highly selective technique of mass spectrometry/gas chromatography with selected ion monitoring. An aqueous solution of LPS was mixed with one of a variety of endodontic irrigants for 30 min. Because it is a commonly used interappointment dressing, calcium hydroxide was also applied to LPS for 1, 2, or 5 days. LPS inactivation was measured by quantitation of free fatty acid release. Water, EDTA, ethanol, 0.12% chlorhexidine, chlorhexidine + sodium hypochlorite, and sodium hypochlorite alone showed little breakdown of LPS. Long-term calcium hydroxide--as well as 30-min exposure to an alkaline mixture of chlorhexidine, ethanol, and sodium hypochlorite--did detoxify LPS molecules by hydrolysis of ester bonds in the fatty acid chains of the lipid A moiety. |
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Authors:
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R A Buck; J Cai; P D Eleazer; R H Staat; H E Hurst |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of endodontics Volume: 27 ISSN: 0099-2399 ISO Abbreviation: J Endod Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-08-03 Completed Date: 2001-10-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7511484 Medline TA: J Endod Country: United States |
Other Details:
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Languages: eng Pagination: 325-7 Citation Subset: D |
Affiliation:
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University of Louisville, KY, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques Calcium Hydroxide / pharmacology* Chromatography, Gas Endotoxins / antagonists & inhibitors*, chemistry Ion-Selective Electrodes Lipid A / analysis, chemistry Lipolysis Lipopolysaccharides / chemistry* Mass Spectrometry Myristic Acids / analysis, chemistry Polysaccharides, Bacterial / drug effects* Root Canal Irrigants / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Endotoxins; 0/Lipid A; 0/Lipopolysaccharides; 0/Myristic Acids; 0/Polysaccharides, Bacterial; 0/Root Canal Irrigants; 1305-62-0/Calcium Hydroxide; 1961-72-4/beta-hydroxymyristic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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