Document Detail

Detoxification of endotoxin by endodontic irrigants and calcium hydroxide.
MedLine Citation:
PMID:  11485249     Owner:  NLM     Status:  MEDLINE    
The effects of endodontic irrigants and calcium hydroxide on lipopolysaccharide (LPS; endotoxin) were analyzed using the highly selective technique of mass spectrometry/gas chromatography with selected ion monitoring. An aqueous solution of LPS was mixed with one of a variety of endodontic irrigants for 30 min. Because it is a commonly used interappointment dressing, calcium hydroxide was also applied to LPS for 1, 2, or 5 days. LPS inactivation was measured by quantitation of free fatty acid release. Water, EDTA, ethanol, 0.12% chlorhexidine, chlorhexidine + sodium hypochlorite, and sodium hypochlorite alone showed little breakdown of LPS. Long-term calcium hydroxide--as well as 30-min exposure to an alkaline mixture of chlorhexidine, ethanol, and sodium hypochlorite--did detoxify LPS molecules by hydrolysis of ester bonds in the fatty acid chains of the lipid A moiety.
R A Buck; J Cai; P D Eleazer; R H Staat; H E Hurst
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endodontics     Volume:  27     ISSN:  0099-2399     ISO Abbreviation:  J Endod     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-08-03     Completed Date:  2001-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  325-7     Citation Subset:  D    
University of Louisville, KY, USA.
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MeSH Terms
Bacteriological Techniques
Calcium Hydroxide / pharmacology*
Chromatography, Gas
Endotoxins / antagonists & inhibitors*,  chemistry
Ion-Selective Electrodes
Lipid A / analysis,  chemistry
Lipopolysaccharides / chemistry*
Mass Spectrometry
Myristic Acids / analysis,  chemistry
Polysaccharides, Bacterial / drug effects*
Root Canal Irrigants / pharmacology*
Reg. No./Substance:
0/Endotoxins; 0/Lipid A; 0/Lipopolysaccharides; 0/Myristic Acids; 0/Polysaccharides, Bacterial; 0/Root Canal Irrigants; 1305-62-0/Calcium Hydroxide; 1961-72-4/beta-hydroxymyristic acid

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