Document Detail


Deterministic separation of cancer cells from blood at 10 mL/min.
MedLine Citation:
PMID:  23112922     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate, propagate and characterize viable CTCs and clusters of cancer cells with their associated stroma cells. Here, we present a microfluidic device for mL/min flow rate, continuous-flow capture of viable CTCs from blood using deterministic lateral displacement (DLD) arrays. We show here that a DLD array device can isolate CTCs from blood with capture efficiency greater than 85% CTCs at volumetric flow rates of up to 10 mL/min with no effect on cell viability.
Authors:
Kevin Loutherback; Joseph D'Silva; Liyu Liu; Amy Wu; Robert H Austin; James C Sturm
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-03
Journal Detail:
Title:  AIP advances     Volume:  2     ISSN:  2158-3226     ISO Abbreviation:  AIP Adv     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101566109     Medline TA:  AIP Adv     Country:  -    
Other Details:
Languages:  ENG     Pagination:  42107     Citation Subset:  -    
Affiliation:
Princeton Institute for the Science and Technology of Materials (PRISM), Princeton Universtiy, Princeton, NJ, USA ; Department of Electrical Engineering, Princeton University, Princeton, NJ, USA.
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