Document Detail

Determining the transgene containment level provided by chloroplast transformation.
MedLine Citation:
PMID:  17420459     Owner:  NLM     Status:  MEDLINE    
Plastids (chloroplasts) are maternally inherited in most crops. Maternal inheritance excludes plastid genes and transgenes from pollen transmission. Therefore, plastid transformation is considered a superb tool for ensuring transgene containment and improving the biosafety of transgenic plants. Here, we have assessed the strictness of maternal inheritance and the extent to which plastid transformation technology confers an increase in transgene confinement. We describe an experimental system facilitating stringent selection for occasional paternal plastid transmission. In a large screen, we detected low-level paternal inheritance of transgenic plastids in tobacco. Whereas the frequency of transmission into the cotyledons of F(1) seedlings was approximately 1.58 x 10(-5) (on 100% cross-fertilization), transmission into the shoot apical meristem was significantly lower (2.86 x 10(-6)). Our data demonstrate that plastid transformation provides an effective tool to increase the biosafety of transgenic plants. However, in cases where pollen transmission must be prevented altogether, stacking with other containment methods will be necessary to eliminate the residual outcrossing risk.
Stephanie Ruf; Daniel Karcher; Ralph Bock
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-09
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  104     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-25     Completed Date:  2007-06-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6998-7002     Citation Subset:  IM    
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany.
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MeSH Terms
Containment of Biohazards / methods*
Genetic Engineering / standards*
Plants, Genetically Modified
Plastids / genetics*
Tobacco / genetics*
Transformation, Genetic*
Comment In:
Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):6879-80   [PMID:  17440039 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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