Document Detail

Determining prevalence of bluetongue and epizootic hemorrhagic disease viruses in mule deer in Arizona (USA) using whole blood dried on paper strips compared to serum analyses.
MedLine Citation:
PMID:  16699159     Owner:  NLM     Status:  MEDLINE    
We investigated the feasibility of using whole blood dried on paper strips as a means to collect antibody prevalence data for the epizootic hemorrhagic disease viruses (EHDV) and bluetongue viruses (BTV) from hunter-harvested male mule deer (Odocoileus hemionus) in October 2002 from Arizona, USA. We compared antibody prevalence estimates in mule deer from paired paper strip and serum samples. Prevalence data obtained from elution of dried blood on paper strips proved to be consistent with results from serum in 94% of the samples tested. The paper strip method allows easy collection of blood from dead animals, with a smaller amount of blood being needed for analyses. Also, samples do not need to be refrigerated before analyses. We also used serum samples to determine hemorrhagic disease (HD) serotype exposure status of mule deer harvested from 4 distinct areas in Arizona. Antibodies to BTV and EHDV were identified in 3 of the 4 areas, with positive results to EHDV-1, EHDV-2, BTV-10, and BTV-11 being most common. Many animals did not have antibodies against the BTV serotypes. Exposure varied geographically and potentially with elevation. Hemorrhagic disease viruses commonly infect Arizona mule deer, except on the Kaibab Plateau in northern Arizona.
Shelli A Dubay; Steven S Rosenstock; David E Stallknecht; James C deVos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of wildlife diseases     Volume:  42     ISSN:  0090-3558     ISO Abbreviation:  J. Wildl. Dis.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-05-15     Completed Date:  2006-07-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0244160     Medline TA:  J Wildl Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  159-63     Citation Subset:  IM    
Arizona Game and Fish Department, 2221 W. Greenway Rd., Phoenix, Arizona 85023, USA.
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MeSH Terms
Arizona / epidemiology
Blood Specimen Collection / methods,  standards,  veterinary
Bluetongue / epidemiology*
Bluetongue virus / immunology*,  isolation & purification
Deer / virology*
Hematologic Tests / methods,  standards,  veterinary
Hemorrhagic Disease Virus, Epizootic / immunology*,  isolation & purification
Reagent Strips
Reoviridae Infections / epidemiology,  veterinary*
Seroepidemiologic Studies
Serotyping / veterinary
Reg. No./Substance:
0/Reagent Strips

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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