| Determining the oxidation states of manganese in PC12 and nerve growth factor-induced PC12 cells. | |
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MedLine Citation:
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PMID: 15964508 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Excessive brain Mn can produce toxicity with symptoms resembling parkinsonism. This syndrome, called "manganism," correlates with loss of dopamine in the striatum and cell death in the striatum and globus pallidus. A common hypothesis is that cell damage in Mn toxicity is caused by oxidation of important cell components by Mn3+. Determination of the amount of Mn3+ present, under a range of conditions, in neuronal cells and brain mitochondria represents an important step in evaluating the "damage through oxidation by Mn3+ hypothesis." In an earlier paper we used X-ray absorption near-edge structure (XANES) spectroscopy to determine the amount of Mn2+ and Mn3+ in brain mitochondria under a range of conditions. Here we extend the study to investigate the evidence for formation of Mn3+ through oxidation of Mn2+ by ROS in PC12 cells and in PC12 cells induced with nerve growth factor (NGF) to display a phenotype more like that of neurons. Although the results suggest that very small amounts of Mn3+ might be present at low Mn levels, probably in Mn superoxide dismutase, Mn3+ is not stabilized by complex formation in these cells and therefore does not accumulate to detectable amounts. |
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Authors:
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Karlene K Gunter; Michael Aschner; Lisa M Miller; Roman Eliseev; Jason Salter; Katie Anderson; Sean Hammond; Thomas E Gunter |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. Date: 2005-03-31 |
Journal Detail:
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Title: Free radical biology & medicine Volume: 39 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-06-20 Completed Date: 2005-10-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 164-81 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, 575 Elmwood Avenue, Rochester, NY 14642, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism Manganese / metabolism Mitochondria / metabolism Nerve Growth Factor / metabolism* Neurons / metabolism Oxidants / metabolism Oxygen / metabolism PC12 Cells Rats Reactive Oxygen Species Spectrometry, X-Ray Emission Spectrophotometry, Atomic / methods Superoxide Dismutase / metabolism Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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R01 ES10041/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oxidants; 0/Reactive Oxygen Species; 7439-96-5/Manganese; 7782-44-7/Oxygen; 9061-61-4/Nerve Growth Factor; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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