Document Detail

Determining the oxidation states of manganese in PC12 and nerve growth factor-induced PC12 cells.
MedLine Citation:
PMID:  15964508     Owner:  NLM     Status:  MEDLINE    
Excessive brain Mn can produce toxicity with symptoms resembling parkinsonism. This syndrome, called "manganism," correlates with loss of dopamine in the striatum and cell death in the striatum and globus pallidus. A common hypothesis is that cell damage in Mn toxicity is caused by oxidation of important cell components by Mn3+. Determination of the amount of Mn3+ present, under a range of conditions, in neuronal cells and brain mitochondria represents an important step in evaluating the "damage through oxidation by Mn3+ hypothesis." In an earlier paper we used X-ray absorption near-edge structure (XANES) spectroscopy to determine the amount of Mn2+ and Mn3+ in brain mitochondria under a range of conditions. Here we extend the study to investigate the evidence for formation of Mn3+ through oxidation of Mn2+ by ROS in PC12 cells and in PC12 cells induced with nerve growth factor (NGF) to display a phenotype more like that of neurons. Although the results suggest that very small amounts of Mn3+ might be present at low Mn levels, probably in Mn superoxide dismutase, Mn3+ is not stabilized by complex formation in these cells and therefore does not accumulate to detectable amounts.
Karlene K Gunter; Michael Aschner; Lisa M Miller; Roman Eliseev; Jason Salter; Katie Anderson; Sean Hammond; Thomas E Gunter
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.     Date:  2005-03-31
Journal Detail:
Title:  Free radical biology & medicine     Volume:  39     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-20     Completed Date:  2005-10-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  164-81     Citation Subset:  IM    
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, 575 Elmwood Avenue, Rochester, NY 14642, USA.
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MeSH Terms
Brain / metabolism
Manganese / metabolism
Mitochondria / metabolism
Nerve Growth Factor / metabolism*
Neurons / metabolism
Oxidants / metabolism
Oxygen / metabolism
PC12 Cells
Reactive Oxygen Species
Spectrometry, X-Ray Emission
Spectrophotometry, Atomic / methods
Superoxide Dismutase / metabolism
Time Factors
Grant Support
Reg. No./Substance:
0/Oxidants; 0/Reactive Oxygen Species; 7439-96-5/Manganese; 7782-44-7/Oxygen; 9061-61-4/Nerve Growth Factor; EC Dismutase

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