Document Detail


Determining the mechanical properties of yeast cell walls.
MedLine Citation:
PMID:  21485033     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The intrinsic cell wall mechanical properties of Baker's yeast (Saccharomyces cerevisiae) cells were determined. Force-deformation data from compression of individual cells up to failure were recorded, and these data were fitted by an analytical model to extract the elastic modulus of the cell wall and the initial stretch ratio of the cell. The cell wall was assumed to be homogeneous, isotropic, and incompressible. A linear elastic constitutive equation was assumed based on Hencky strains to accommodate the large stretches of the cell wall. Because of the high compression speed, water loss during compression could be assumed to be negligible. It was then possible to treat the initial stretch ratio and elastic modulus as adjustable parameters within the analytical model. As the experimental data fitted numerical simulations well up to the point of cell rupture, it was also possible to extract cell wall failure criteria. The mean cell wall properties for resuspended dried Baker's yeast were as follows: elastic modulus 185 ± 15 MPa, initial stretch ratio 1.039 ± 0.006, circumferential stress at failure 115 ± 5 MPa, circumferential strain at failure 0.46 ± 0.03, and strain energy per unit volume at failure 30 ± 3 MPa. Data on yeast cells obtained by this method and model should be useful in the design and optimization of cell disruption equipment for yeast cell processing. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011.
Authors:
John D Stenson; Peter Hartley; Changxiang Wang; Colin R Thomas
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Publication Detail:
Type:  Journal Article     Date:  2011-02-28
Journal Detail:
Title:  Biotechnology progress     Volume:  27     ISSN:  1520-6033     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-04-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  505-12     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 American Institute of Chemical Engineers (AIChE).
Affiliation:
School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, U.K.
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