Document Detail


Determining of actual activities of acid and alkaline ribonuclease in human serum and urine.
MedLine Citation:
PMID:  1842595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acid and alkaline ribonuclease (RNase) activities were measured in serum and urine using procedure based on assumption that all determined RNase activities, both at pH 6.5 and 7.8 represent values produced by overlapping of activities of acid leukocyte type RNase and alkaline pancreatic type RNase. The procedure requires simultaneous determining of RNase activity at pH 6.5 and 7.8 and further calculation of actual activities of acid and alkaline RNase activities using the elaborated experimental formula. Results of determining acid and alkaline RNases in human sera yielded on information on specific contribution of leukocyte type and pancreatic type RNases to increased RNase activity in such clinical conditions as terminal renal failure, myocardial infarction and chronic myelogenous leukemia. It was also found that there is in human urine a remarkably increased proportion of acid RNase activity if compared to this in serum.
Authors:
J W Naskalski; A Celiński
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Materia medica Polona. Polish journal of medicine and pharmacy     Volume:  23     ISSN:  0025-5246     ISO Abbreviation:  Mater Med Pol     Publication Date:    1991 Apr-Jun
Date Detail:
Created Date:  1993-01-14     Completed Date:  1993-01-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0236526     Medline TA:  Mater Med Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  107-10     Citation Subset:  IM    
Affiliation:
Department of Biochemical Diagnostics, Medical Academy, Kraków, Poland.
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MeSH Terms
Descriptor/Qualifier:
Humans
Ribonucleases / analysis*,  blood,  urine
Chemical
Reg. No./Substance:
EC 3.1.-/Ribonucleases

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