Document Detail

Determination of zinc diethyldithiocarbamate released into artificial sweat from natural rubber latex vulcanizates by HPLC.
MedLine Citation:
PMID:  17254376     Owner:  NLM     Status:  MEDLINE    
A simple high-performance liquid chromatography method is adopted in order to quantitate the amount of zinc diethyldithiocarbamate (ZDEC) released into artificial sweat from natural rubber latex vulcanizates. The artificial sweat is extracted with dichloromethane, and the residue is recovered and re-dissolved in a known quantity of dichloromethane. ZDEC is quantitated as its copper complex by reacting with copper(II) sulphate. A reversed-phase C18 column and detection wavelength of 435 nm are used to measure the copper-dithiocarbamate complex. The procedure is repeated with cobalt(II) chloride, and the amount of ZDEC obtained by both the methods is compared. It is found that the recovery of ZDEC from the artificial sweat is high when copper(II) sulphate is used, indicating that the copper(II) sulphate is a better complexing agent than cobalt(II) chloride under the conditions used in the present study. The limits of detection and the quantitation of ZDEC are found to be 0.25 and 0.86 microg/mL, respectively. The present method, based on precolumn derivatization using copper(II) sulphate, facilitates the quantitation of ZDEC in latex products.
Elizabeth K Abraham; P Ramesh; R Joseph
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  45     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-26     Completed Date:  2007-04-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram-695 012, Kerala, India.
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MeSH Terms
Chelating Agents / chemistry
Chromatography, High Pressure Liquid / methods*
Cobalt / chemistry
Copper Sulfate / chemistry
Ditiocarb / analysis*,  chemistry
Occupational Exposure / analysis
Rubber / chemistry*
Sweat / chemistry*
Reg. No./Substance:
0/Chelating Agents; 147-84-2/Ditiocarb; 7440-48-4/Cobalt; 7646-79-9/cobaltous chloride; 7758-98-7/Copper Sulfate; 9006-04-6/Rubber

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