| Determination of zeta-potential in rat organotypic hippocampal cultures. | |
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MedLine Citation:
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PMID: 18263658 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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zeta-potentials of entities such as cells and synaptosomes have been determined, but zeta of brain tissue has never been measured. Electroosmotic flow, and the resulting transport of neuroactive substances, would result from naturally occurring and experimentally or clinically induced electric fields if zeta is significant. We have developed a simple method for determining zeta in tissue. An electric field applied across a rat organotypic hippocampal slice culture (OHSC) drives fluorescent molecules through the tissue by both electroosmotic flow and electrophoresis. Fluorescence microscopy is used to determine each molecule's velocity. Independently, capillary electrophoresis is used to measure the molecules' electrophoretic mobilities. The experiment yields zeta-potential and average tissue tortuosity. The zeta-potential of OHSCs is -22 +/- 2 mV, and the average tortuosity is 1.83 +/- 0.06. In a refined experiment, zeta-potential is measured in various subregions. The zeta-potentials of the CA1 stratum pyramidale, CA3 stratum pyramidal, and dentate gyrus are -25.1 +/- 1.6 mV, -20.3 +/- 1.7 mV, and -25.4 +/- 1.0 mV, respectively. Simple dimensional arguments show that electroosmotic flow is potentially as important as diffusion in molecular transport. |
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Authors:
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Yifat Guy; Mats Sandberg; Stephen G Weber |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-02-08 |
Journal Detail:
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Title: Biophysical journal Volume: 94 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-15 Completed Date: 2008-06-10 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 4561-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport, Active / physiology* Cells, Cultured Diffusion Electromagnetic Fields Hippocampus / chemistry*, physiology* Microscopy, Fluorescence / methods* Rats |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM 44842/GM/NIGMS NIH HHS |
| Comments/Corrections | |
Erratum In:
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Biophys J. 2008 Oct;95(7):3521 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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