| Determination of zafirlukast by stability indicating LC and derivative spectrophotometry. | |
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MedLine Citation:
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PMID: 12367695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two analytical methods have been developed for the determination of zafirlukast, a novel anti-asthmatic drug: high performance liquid chromatography (HPLC) and derivative spectrophotometry (DS). HPLC with ultraviolet detection at 225 nm is carried out with a Symmetry Shield RP18 column and a mobile phase constituted of acetonitrile and 0.01 M potassium dihydrogen phosphate buffer, adjusted the pH to 3.5 with 0.1 M KOH. The LC method is simple, rapid, selective and stability indicating. Indole was used as internal standard for the purpose of quantification of zafirlukast in HPLC. Spectrophotometry uses the third order derivative of the UV spectrum at 251.1 nm (deltalambda value 2.1 nm) for determination. Both methods were fully validated and a comparison was made. The results confirm that the methods are highly suitable for its intended purpose. |
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Authors:
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T Radhakrishna; J Satyanarayana; A Satyanarayana |
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Publication Detail:
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Type: Comparative Study; Journal Article; Validation Studies |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 30 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-10-07 Completed Date: 2003-04-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 695-703 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Elsevier Science B.V. |
Affiliation:
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Department of Physical Chemistry, School of Chemistry, Andhra University, Visakhapatnam-530003, India. a_s_narayana@rediffmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid
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methods Chromatography, Liquid / methods Drug Stability Spectrophotometry, Ultraviolet / methods Tosyl Compounds / analysis*, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Tosyl Compounds; 107753-78-6/zafirlukast |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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