Document Detail

Determination of vitamin A in foods--a review.
MedLine Citation:
PMID:  336289     Owner:  NLM     Status:  MEDLINE    
Formerly, few foods were routinely analyzed for vitamin A, but recent emphasis on nutrient requirements, nutrient labeling, and use of dietary convenience foods has created need for determining vitamin A in a variety of foods. There are many vitamin A methods--some suitable for certain products only. For regulatory purposes, the FDA specifies the AOAC method where it is applicable. However, some food analysts and organizations continue with their own vitamin A methods. If possible, a single, widely applicable general method should be used for vitamin A in foods. Vitamin A may be determined by spectrophotometric, colorimetric, and fluorometric procedures. Sometimes chromatography is required as an important part of the method. Colorimetric procedures with SbCl3 are now most widely used to measure vitamin A (retinol) in foods. If vitamin A content is high enough and extracts sufficiently free of interfering substances, spectrophotometric or flurometric methods are satisfactory. Methods in various stages of development for determining vitamin A in foods are based on flurospectrophotometry, gas-liquid chromatography, high-performance liquid chromatography, and automation. To estimate total vitamin A nutritional value of certain foods may also require determination of vitamin A isomerization and contents of carotenes, cryptoxanthin, reinaldehyde, and apo-carotenal.
D B Parrish
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  CRC critical reviews in food science and nutrition     Volume:  9     ISSN:  0099-0248     ISO Abbreviation:  CRC Crit Rev Food Sci Nutr     Publication Date:  1977  
Date Detail:
Created Date:  1978-01-27     Completed Date:  1978-01-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7513255     Medline TA:  CRC Crit Rev Food Sci Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  375-94     Citation Subset:  IM    
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MeSH Terms
Animal Feed / analysis
Carotenoids / analysis*
Cereals / analysis
Chromatography / methods
Colorimetry / methods
Dairy Products / analysis
Evaluation Studies as Topic
Fluorometry / methods
Food Analysis / methods*
Food, Formulated / analysis
Food, Fortified / analysis
Infant Food / analysis
Margarine / analysis
Meat / analysis
Vitamin A / analysis*
Reg. No./Substance:
11103-57-4/Vitamin A; 36-88-4/Carotenoids; 8029-82-1/Margarine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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