Document Detail

Determination of vitamin K1 isomers in foods by liquid chromatography with C30 bonded-phase column.
MedLine Citation:
PMID:  12083261     Owner:  NLM     Status:  MEDLINE    
Vitamin K1 was determined in a variety of foods by using reversed-phase liquid chromatography with a C30 column followed by post-column reduction to the fluorescent hydroquinone derivatives. Lipids were removed by lipase digestion, followed by single extraction into hydrocarbon, and the protocol was extended to selected natural and processed foods. Biologically active trans- and inactive ci-vitamin K1 isomers were measured individually to evaluate the true nutritional status of the products. Method performance parameters confirmed the validity of the technique. The use of the triacontyl-bonded C30 phase for selective phylloquinone isomer measurement extends previously validated AOAC Method 999.15 for vitamin K1 in milk and infant formula to a wider range of foods important in the human diet. The cis-vitamin K1 isomer contributes up to about 15% of total phylloquinone in certain foods.
David C Woollard; Harvey E Indyk; Bertram Y Fong; Kathleen K Cook
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  85     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2002 May-Jun
Date Detail:
Created Date:  2002-06-26     Completed Date:  2002-12-12     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  682-91     Citation Subset:  IM    
AgriQuality NZ Ltd, Auckland, New Zealand.
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MeSH Terms
Chromatography, Liquid / methods*
Food Analysis / methods*
Vitamin K 1 / analysis*
Reg. No./Substance:
84-80-0/Vitamin K 1

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