Document Detail

Determination of vitamin B12 in infant formula and adult nutritionals by surface plasmon resonance: First Action 2011.16 (test kit method).
MedLine Citation:
PMID:  22649915     Owner:  NLM     Status:  In-Process    
At the "Standards Development and International Harmonization: AOAC INTERNATIONAL Mid-Year Meeting" on June 29, 2011, an Expert Review Panel (ERP) agreed to further examine AOAC Official Method 2011.01, "Determination of Vitamin B12 by Surface Plasmon Resonance," for use with infant formula and adult nutritionals. The original collaborative study was conducted using the Biacore Q biosensor instrument and the Biacore Q Qflex Kit Vitamin B12 PI. Samples included in the study were infant formula, cereals, premixes, vitamin tablets, dietary supplements, and baby food. Eleven laboratories participated in the collaborative study. The results demonstrated a repeatability RSD (RSDr) of 1.59-27.8 and HorRat values for reproducibility of 0.34-1.89 in samples with levels ranging from ppm to ppb. The assay studied is a label-free protein binding-based assay that uses the principle of surface plasmon resonance to measure the interaction between vitamin B12 and a specific binding protein by passing a portion of the prepared sample extract combined with binding protein solution across a functionalized sensor chip. The response from the functionalized sensor chip is given as free-binding protein, as the mixture binds to the prepared surface of the chip. The ready-to-use Qflex Kit Vitamin B12 PI provides the reagents and accessories necessary to perform this assay. AOAC Method 2011.01 was approved by the AOAC Method Committee on Food Nutrition for Official First Action status, applicable to a wide range of food products, dietary supplements, and multivitamin premixes. After evaluation of the validation data available, an ERP agreed in June 2011 that the method meets standard method performance requirements, as articulated by the Stakeholder Panel on Infant Formula and Adult Nutritionals. The ERP granted the method First Action status, applicable to infant formula and adult/pediatric nutritional formula.
Pathik Vyas; Anthony A O'Kane; Dawn Dowell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  95     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2012 Mar-Apr
Date Detail:
Created Date:  2012-06-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  329-34     Citation Subset:  IM    
AsureQuality Auckland Laboratory, 131 Boundary Rd, Blockhouse Bay, Auckland, New Zealand.
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