Document Detail

Determination of vitamin B12 in food products by liquid chromatography/UV detection with immunoaffinity extraction: single-laboratory validation.
MedLine Citation:
PMID:  18727538     Owner:  NLM     Status:  MEDLINE    
A fast and simple method to determine vitamin B12 in foods is presented. The method allows, in addition to the determination of added cyanocobalamin, the determination of natural vitamin B12 forms, making it also applicable to nonfortified products, especially those that are milk-based. Vitamin B12 is extracted in sodium acetate buffer in the presence of sodium cyanide (100 degrees C, 30 min). After purification and concentration with an immunoaffinity column, vitamin B12 is determined by liquid chromatography with UV detection (361 nm). The method has been validated in analyses of a large range of products: milk- and soy-based infant formulas, cereals, cocoa beverages, health care products, and polyvitamin premixes. The method showed appropriate performance characteristics: linear response over a large range of concentrations, recovery rates of 100.8 +/- 7.5% (average +/- standard deviation), relative standard deviation of repeatability, RSDr, of 2.1%, and intermediate reproducibility, RSDiR, of 4.3%. Limits of detection and quantitation were 0.10 and 0.30 microg/100 g, respectively, and correlation with the reference microbiological assay was good (R2 = 0.9442). The proposed method is suitable for the routine determination of vitamin B12 in fortified foods, as well as in nonfortified dairy products. It can be used as a faster, more selective, and more precise alternative to the classical microbiological determination.
Esther Campos-Giménez; Patric Fontannaz; Marie-Jose Trisconi; Tamara Kilinc; Catherine Gimenez; Pierre Andrieux
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  91     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2008 Jul-Aug
Date Detail:
Created Date:  2008-08-27     Completed Date:  2008-09-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  786-93     Citation Subset:  IM    
Nestlé Research Center, Vers-chez-les-blanc, CH-1000 Lausanne 26, Switzerland.
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MeSH Terms
Chromatography, Affinity
Chromatography, High Pressure Liquid
Food, Fortified / analysis*
Indicators and Reagents
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet
Vitamin B 12 / analysis*
Vitamins / analysis*
Reg. No./Substance:
0/Indicators and Reagents; 0/Solvents; 0/Vitamins; 68-19-9/Vitamin B 12

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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