| Determination of urinary and salivary cotinine using gas and liquid chromatography and enzyme-linked immunosorbent assay. | |
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MedLine Citation:
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PMID: 11888058 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to compare cotinine concentrations in urine and saliva using gas chromatography (GC), high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). Ninety-four subjects were selected (27 smokers and 67 non-smokers) and interviewed using questionnaire. Of the non-smokers, 39 had been exposed to environmental tobacco smoke (ETS) and 28 had not been exposed to ETS. Cotinine levels among smokers were highest using all three measurements, followed by ETS exposed subjects and non-smokers. Cotinine levels in urine, using HPLC, correlated significantly with levels measured using ELISA (r=0.92) and GC-nitrogen-phosphorus detection (NPD) (r=0.92). Salivary cotinine levels measured using ELISA did not correlate significantly with either HPLC (r=0.37) or GC-NPD (r=0.33) measurements. Multiple regression models were used to adjust for age, gender, drug use and health status, and it was found that cotinine levels in urine and saliva were significantly correlated with smoking pack-year. The authors conclude that urinary cotinine concentration is a more accurate biomarker for ETS than salivary cotinine concentration. |
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Authors:
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H W Kuo; J S Yang; M C Chiu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 768 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-03-12 Completed Date: 2002-08-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 297-303 Citation Subset: IM |
Affiliation:
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Institute of Environmental Health, China Medical College, Taichung, Taiwan. wukuo@mail.cmc.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Case-Control Studies Chromatography, Gas / methods* Chromatography, High Pressure Liquid / methods* Cotinine / analysis*, urine Enzyme-Linked Immunosorbent Assay / methods* Female Humans Male Saliva / chemistry* Smoking |
| Chemical | |
Reg. No./Substance:
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486-56-6/Cotinine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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