Document Detail


Determination of uric acid in human urine and serum by capillary electrophoresis with chemiluminescence detection.
MedLine Citation:
PMID:  18445465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and sensitive method based on capillary electrophoresis (CE) with chemiluminescence (CL) detection has been developed for the determination of uric acid (UA). The sensitive detection was based on the enhancement effect of UA on the CL reaction between luminol and potassium ferricyanide (K3[Fe(CN)6]) in alkaline solution. A laboratory-built reaction flow cell and a photon counter were deployed for the CL detection. Experimental conditions for CL detection were studied in detail to achieve a maximum assay sensitivity. Optimal conditions were found to be 1.0 x 10(-4) M luminol added to the CE running buffer and 1.0 x 10(-4) M K3[Fe(CN)6] in 0.2 M NaOH solution introduced postcolumn. The proposed CE-CL assay showed good repeatability (relative standard deviation [RSD]=3.5%, n=11) and a detection limit of 3.5 x 10(-7) M UA (signal/noise ratio [S/N]=3). A linear calibration curve ranging from 6.0 x 10(-7) to 3.0 x 10(-5) M UA was obtained. The method was evaluated by quantifying UA in human urine and serum samples with satisfactory assay results.
Authors:
Shulin Zhao; Jianshi Wang; Fanggui Ye; Yi-Ming Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-10
Journal Detail:
Title:  Analytical biochemistry     Volume:  378     ISSN:  1096-0309     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-09     Completed Date:  2008-07-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  127-31     Citation Subset:  IM    
Affiliation:
College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com
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MeSH Terms
Descriptor/Qualifier:
Borates / pharmacology
Electrophoresis, Capillary / methods*
Ferricyanides / pharmacology
Humans
Luminescence*
Luminol / pharmacology
Reference Standards
Sodium Hydroxide / pharmacology
Solutions
Uric Acid / blood*,  urine*
Chemical
Reg. No./Substance:
0/Borates; 0/Ferricyanides; 0/Solutions; 1310-73-2/Sodium Hydroxide; 13746-66-2/potassium ferricyanide; 521-31-3/Luminol; 69-93-2/Uric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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